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pFastBac1_GI.1_N116C-G193C

Plasmid

#162580

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Asparagine 116 was mutated to Cysteine and Glycin…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGV3319

Plasmid

#170277

Purpose

Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

Bst-LF D720A

Tags

Hexahistidine

Expression

Bacterial

Mutation

D720A

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:cAPX

Plasmid

#84736

Purpose

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

APX1 (APX1 Mustard Weed)
HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-NRBP1

Plasmid

#48197

Purpose

Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

Depositor

Joan Conaway, Ronald Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

NRBP1 (NRBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-PFDN2

Plasmid

#202664

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

prefoldin subunit 2 (PFDN2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Nuc-mTurq2-Apollo-NADP+

Plasmid

#193302

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

SV40 NLS and mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-Alpha-V-Integrin-25

Plasmid

#55465

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3

Plasmid

#173936

Purpose

Bacterial expression of PARP1 lacking Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(cd226)

Plasmid

#177900

Purpose

Bacterial expression of his10-tagged human cd226 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CD226 (aa 288-336 only) (CD226 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA

Plasmid

#154776

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in GFP reporter, hybrid PylT with A…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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