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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36,031 results for: CaS;

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Showing: 10281 - 10300 of 36031 results

  1. pZFX-donor

    Plasmid
    #112366
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFX with GFP
    Depositor
    Kevin White
    Insert
    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    Use
    CRISPR
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112366-plasmid-map-sequence-id-229714

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  2. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  3. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Promoter
    G. gallus U6.3
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  4. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Alejandro Chavez
    Insert
    PE2
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158567-plasmid-map-sequence-id-309882

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  5. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

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  6. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177232-plasmid-map-sequence-id-363978

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  7. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  8. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177235-plasmid-map-sequence-id-352696

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  9. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

    Plasmid
    #177231
    Purpose
    Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgNeo2
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177231-plasmid-map-sequence-id-352660

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  11. ipUSEPR-sg-Hs-PCNA

    Plasmid
    #188686
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPCNA (PCNA Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188686-plasmid-map-sequence-id-375116

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  12. pPITX2-donor

    Plasmid
    #176372
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    PITX2 (PITX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176372-plasmid-map-sequence-id-347400

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  13. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

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  14. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Mutation
    D10A nickase
    Promoter
    T7
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

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  15. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

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  16. bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

    Plasmid
    #177236
    Purpose
    Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgSnrk
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177236-plasmid-map-sequence-id-352699

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  17. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid
    #177234
    Purpose
    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNuak1/sgNuak2
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177234-plasmid-map-sequence-id-352695

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  18. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177233-plasmid-map-sequence-id-352662

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  19. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd

    Plasmid
    #177230
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgLkb1_2nd
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177230-plasmid-map-sequence-id-352659

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  20. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

    Plasmid
    #177227
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgLkb1_1st
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177227-plasmid-map-sequence-id-352658

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Showing: 10281 - 10300 of 36031 results