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pMEH371 CD63 HA

Plasmid

#168220

Purpose

Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag

Depositor

Joshua Leonard

Publication

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

CD63 (CD63 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLPC-Flag-SOCS1

Plasmid

#129514

Purpose

Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

Depositor

Gerardo Ferbeyre

Publication

Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

Insert

SOCS1 (SOCS1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVL-6

Plasmid

#104584

Purpose

Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

Depositor

Daniel Christ

Publication

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)

Expression

Mammalian

Mutation

L234A/L235E/G237A and K322A/P331S

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC

Plasmid

#99641

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Publication

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pcFLAG-CSK-WT

Plasmid

#74502

Purpose

Overexpression of CSK in mammalian cells

Depositor

Lars Rönnstrand

Publication

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 Utr230-EGFP

Plasmid

#58472

Purpose

Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Utr230 (UTRN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQHA-USP7 CS puroR

Plasmid

#46754

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMA3287

Plasmid

#46883

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Publication

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase, Y147A mutant (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Y147A

Promoter

TRE-Tight

Available since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1 JellyOp

Plasmid

#41432

Depositor

Robert Lucas

Publication

Bailes et al PLoS One. 2012;7(1):e30774. Epub 2012 Ja

Insert

Box jellyfish opsin

Tags

1D4 epitope at 3' end

Expression

Mammalian

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p1990 SV40-LMP1

Plasmid

#26654

Depositor

Bill Sugden

Publication

Kaykas et al EMBO J. 2001 Jun 1. 20(11):2641-54.

Insert

Latent Membrane Protein 1 (LMP-1 Epstein-Barr Virus)

Expression

Mammalian

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

Plasmid

#30505

Depositor

Ichiro Matsumura

Publication

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Tags

His

Expression

Bacterial

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Publication

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Available since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only

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mADAM10 deltaMP in pQCXIP cFLAG

Plasmid

#19138

Depositor

Zena Werb

Publication

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 (Adam10 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 56-457

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGST-PPARdelta

Plasmid

#16548

Depositor

Bert Vogelstein

Publication

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta (PPARD Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-249

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAd-Track SIRT1 H355A

Plasmid

#8441

Depositor

Pere Puigserver

Publication

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pMX GFP cdc42 Q61L

Plasmid

#14568

Depositor

Joan Brugge

Publication

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

cdc42 Q61L (CDC42 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Publication

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

Y261A

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
HA.PKD.Y463E

Plasmid

#10812

Depositor

Alex Toker

Publication

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

Y463E--active; P467L and L520P

Promoter

CMV

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles