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pGP-CMV-jGCaMP7b

Plasmid

#104484

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7b

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-As-Cas12a-2xNLS

Plasmid

#155047

Purpose

Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

AsCas12a

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS, Myc-tag and SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/15R]

Plasmid

#225376

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat) IgG2a R-mAb. Derived from hybridoma N110/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neuroligin-3 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Nlgn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/ZMYM2-FGFR1

Plasmid

#167486

Purpose

Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

Depositor

Nicholas Cross

Article

Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

Insert

ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N425/45]

Plasmid

#190326

Purpose

Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/4/8 [N245/36R]

Plasmid

#177522

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/4/8 (Rat). Derived from hybridoma N245/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody (Tarp Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/8R]

Plasmid

#177572

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-S-tag [N56/9R]

Plasmid

#206600

Purpose

Mammalian Expression Plasmid of anti-S-tag (Bovine). Derived from hybridoma N56/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-S-tag (Bos taurus) recombinant Mouse monoclonal antibody (RNASE1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-DreO-bGHpA

Plasmid

#50363

Purpose

Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV Retrograde and AAV5

Insert

DreO recombinase

Use

AAV

Tags

none

Mutation

Sequence optimized for expression in mammalian ce…

Promoter

hSyn1

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHRIG-Akt3

Plasmid

#53596

Purpose

lentiviral expression of constitutively active human Akt3 and EGFP

Depositor

Heng Zhao

Article

Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

Insert

cAkt3 (AKT3 Human)

Use

Lentiviral

Tags

HA and IRES-EGFP

Expression

Mammalian

Mutation

constitutively active (myristoylated version)

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Stx5S-mCherry

Plasmid

#165087

Purpose

Expresses Stx5S-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Short isoform) (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacks amino acids 1-54, enocdes short isoform of …

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-WT Neo

Plasmid

#223058

Purpose

METTL1 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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rag2:myr-Akt2_pISceI

Plasmid

#231495

Purpose

Expresses myristolated Akt2 in zebrafish lymphoid and mesenchymal cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

Akt2 (Akt2 Mouse)

Use

Zebrafish expression

Tags

N-terminal myristolation signal

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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Slc17a7-IRES2-Cre targeting vector

Plasmid

#61574

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Slc17a7-IRES2-Cre

Use

Cre/Lox and Mouse Targeting

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-VDAC1 [N152B/23R-2b]

Plasmid

#222171

Purpose

Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody. (VDAC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

Plasmid

#115694

Purpose

All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Inserts

NmeCas9
Nme-sgRNA

Use

CRISPR

Tags

NLS and NLS, HA

Expression

Insect and Mammalian

Mutation

None and fusion of repeat/tracrRNA to make a sgRNA

Promoter

EF-1 alpha and U6

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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