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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

Plasmid

#192160

Purpose

Dual AAV vector AAV-dCas9

Depositor

Sidi Chen

Publication

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Insert

Dead Cas9

Use

AAV

Mutation

NA

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero11

Plasmid

#187928

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero11

Depositor

Yukihide Tomari

Publication

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero11 (C19orf53 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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OA-986F (AAEL003877-dCas9-VPR)

Plasmid

#183993

Purpose

Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

Depositor

Omar Akbari

Publication

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

AAEL003877-dCAS9-VPR

Use

CRISPR

Tags

dCas9-VPR

Expression

Insect

Promoter

AAEL003887

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

Plasmid

#177232

Purpose

Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik1_1st/sgSik2/sgSik3_1st

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Publication

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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1137=Hsp70Bb-Cas9[dsRed]

Plasmid

#149424

Purpose

The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

Depositor

Omar Akbari

Publication

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

Cas9

Use

CRISPR

Tags

eGFP

Expression

Insect

Promoter

Dmel Hsp70Bb

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Publication

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0013

Plasmid

#117521

Purpose

Level0 Golden Gate part: CDS, SpCas9-p

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-p (with stop codon)

Use

CRISPR

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-tvD-Cit-tevs-DHFR

Plasmid

#116054

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Publication

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tvD-Cit-tevs-DHFR

Expression

Mammalian

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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LZF80: Cre-on CAG-somCheRiff-CFP (DIO)

Plasmid

#104115

Purpose

synOptopatch construct

Depositor

Adam Cohen

Publication

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

somCheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only
E. coli mRNA toehold switch sensor

Plasmid

#110708

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Publication

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AGPAT3_pCSdest

Plasmid

#53928

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

AGPAT3 (AGPAT3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
ERG_pCSdest

Plasmid

#53864

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ERG (ERG Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
DSCR3_pCSdest

Plasmid

#53874

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DSCR3 (VPS26C Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
IFNGR2_pCSdest

Plasmid

#53823

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IFNGR2 (IFNGR2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
ITSN1_pCSdest

Plasmid

#53800

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Publication

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

Plasmid

#89886

Purpose

Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Publication

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-NRasQ61K

Use

Unspecified

Tags

mCherry

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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