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pKP1583

Plasmid

#167645

Purpose

Express FepA D34C . Please note that mutation numbers are not based on start codon of insert.

Depositor

Kathleen Postle

Article

Postle et al bioRxiv 2021.12.08.471779

Insert

fepA D34C

Use

Tags

Expression

Bacterial

Mutation

fepA with D34C mutation (Mutation is D56C based o…

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV_puro_41589

Plasmid

#111632

Purpose

Complementation of Dicer_KO mESCs with mutated human DICER isoform

Depositor

Constance Ciaudo

Insert

FLAG-hsDicer (K70A) (DICER1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K70A

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0158

Plasmid

#154639

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

NOS (C-CRE relocated) Firefly luciferase expression cassette [NOS (C-CRE relocated)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1584

Plasmid

#139875

Purpose

Expression plasmid of rings 2 and 3 of LetB for crystallography

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(159-383)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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D68_Ld_161580

Plasmid

#135881

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMJA158

Plasmid

#128533

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAW158

Plasmid

#113177

Purpose

E. coli expression vector for untagged E. coli Cas1 R112E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R112E

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPN158

Plasmid

#91687

Purpose

Express sgRNA targeting human ZSWIM6

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

ZSWIM6 (ZSWIM6 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3158

Plasmid

#41332

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ryr2b-Left (ryr2b Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1580

Plasmid

#29266

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple109 (HBEGF Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1587

Plasmid

#29268

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple116 (HSPA12B Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1583

Plasmid

#29267

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple112 (HCRT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pJ158

Plasmid

#29492

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf158

Plasmid

#12846

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jon Yewdell, Jack Bennink

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf158

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
GPR158-Tango

Plasmid

#66332

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR158 (GPR158 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEJS2158_Nme2-ABE8e-i1

Plasmid

#201510

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HIR Y1158,1162,1163F

Plasmid

#24054

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Y1158F, Y1162F, Y1163F

Promoter

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
SIRT5H158Y-pET15b

Plasmid

#81032

Purpose

Bacterial expression of Human catalytic mutant SIRT5 protein

Depositor

David Lombard

Article

Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

Insert

Sirt5 (SIRT5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H158Y catalytic mutant

Promoter

T7

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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HIR Y1158F

Plasmid

#24051

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Y1158F

Promoter

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
KA1158_pPBCAG-MerCreMer-IZ

Plasmid

#124184

Purpose

piggyBac-based expression of the tamoxifen-inducible Cre

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

MerCreMer-IRES-Zeo

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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