We narrowed to 1,528 results for: Flp

1. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn FLEx-FRT hM3Dq-mCherry

   Plasmid

   #161575

   Purpose

   Expression of hM3Dq-mCherry in a Flp-dependent fashion

   Depositor

   Robert Malenka, Kevin Beier

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT-TO-DEST

   Plasmid

   #106348

   Purpose

   Destination vector for gateway cloning. Developed for Flp-In T-REx system.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Type

   Empty backbone

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5B-FRT-AP-Sstr3-GFP-DEST

   Plasmid

   #49098

   Purpose

   Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Sstr3 (Sstr3 Mouse)
   

   Tags

   GFP and acceptor peptide (AP)

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

   Plasmid

   #90003

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

   Plasmid

   #90008

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

   Plasmid

   #90004

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

   Plasmid

   #90009

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

   Plasmid

   #203839

   Purpose

   Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dCre
   

   Use

   AAV

   Tags

   mScarlet-KASH-2A

   Mutation

   Enzymatically inactive Cre

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

    Plasmid

    #195536

    Purpose

    Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

    Plasmid

    #113549

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid

    #52890

    Purpose

    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Inserts

    LexGAD
    Gal4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FDIO-IGK-dAPEX2-KDEL

    Plasmid

    #117184

    Purpose

    Plasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-mCherry-N

    Plasmid

    #210271

    Purpose

    N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP

    Plasmid

    #136005

    Purpose

    DOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-term of G3BP1 Deleted for Delta RBP

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-CAG-F14F15S-BFP2-(mCherry)'

    Plasmid

    #115235

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Expression

    Mammalian

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. B9L-BirA*

    Plasmid

    #109146

    Purpose

    To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    B9L (Bcl9l Mouse)
    

    Tags

    BirA(R118G) and Flag

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO/SH/GW-Mst1

    Plasmid

    #84299

    Purpose

    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.

    Depositor

    Jonathan Chernoff

    Article

    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

    Insert

    Mst1 (STK4 Human)
    

    Tags

    HA, SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

20. EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

    Plasmid

    #236148

    Purpose

    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    E1b minimal promoter with the UTRN promoter region

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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