We narrowed to 11,219 results for: NSI;

1. pBabe-tetoff-mVenus-PICdc42

   Plasmid

   #91876

   Purpose

   PI(WT): Photo inhibitable (blue light sensitive)

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Vav2 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1_tdTomato

   Plasmid

   #198041

   Purpose

   Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Insert

   tdTomato
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a isPETase

   Plasmid

   #225759

   Purpose

   Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   isPETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS159

   Plasmid

   #122378

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-VinculinTS

   Plasmid

   #111830

   Purpose

   Vinculin tension sensor in lentiviral expression vector

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGreen_dualluc_ORF_sensor

   Plasmid

   #55207

   Purpose

   Dual-luciferase reporter for microRNA targeting in plants

   Depositor

   Michael Axtell

   Article

   Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

   Inserts

   Renilla luciferase
   Firefly luciferase
   

   Use

   Luciferase; Plant transient expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEXP5-NT/pT7 LasI

   Plasmid

   #193631

   Purpose

   Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LasI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS143

   Plasmid

   #122377

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEXP5-NT/pT7 LuxI

   Plasmid

   #193626

   Purpose

   Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LuxI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ykgMO-IGS-sfGFP

    Plasmid

    #207231

    Purpose

    Calprotectin sensing promoter with sfGFP reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TS module

    Plasmid

    #26021

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Tension Sensor module
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEXP5-NT/pT7 LuxR

    Plasmid

    #193625

    Purpose

    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LuxR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEXP5-NT/pT7 LasR

    Plasmid

    #193629

    Purpose

    Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LasR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. VinculinTS-A50I

    Plasmid

    #111827

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS263

    Plasmid

    #122379

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-ro1GFP

    Plasmid

    #82369

    Purpose

    Expression of redox sensitive GFP in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C48S/F64L/T65S/S147C/Q204C

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. myr-palm_mCherry2-mEGFP

    Plasmid

    #127816

    Purpose

    Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS267

    Plasmid

    #122380

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge