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pEMS2058

Plasmid

#49136

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1978

Plasmid

#49110

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre-WPRE

Use

AAV

Expression

Mammalian

Promoter

C8ORF46

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2026

Plasmid

#49133

Purpose

AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple301-iCre

Use

AAV

Expression

Mammalian

Promoter

TNNT1-B

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2386

Plasmid

#225639

Purpose

AAV plasmid with Ple388 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

AAV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pT22gCfpUbiG4FER4P2A(#249)

Plasmid

#184070

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

Gal4bdFF-ERT2-P2A

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL33

Plasmid

#135103

Purpose

Codon-optimized HSV-1 UL33 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL50-CStHA

Plasmid

#135105

Purpose

Codon-optimized HSV-1 UL50 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL33-CStHA

Plasmid

#135106

Purpose

Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUS2-CStHA

Plasmid

#135107

Purpose

Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Tags

Strep-Strep-HA

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2235

Plasmid

#111888

Purpose

High copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

Unspecified

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2236

Plasmid

#111889

Purpose

High copy number plasmid containing Ple321 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

Unspecified

Promoter

Ple321 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2025

Plasmid

#49132

Purpose

AAV plasmid with NOV (Ple303) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple303-iCre

Use

AAV

Expression

Mammalian

Promoter

NOV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Article

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
TetO-FUW-NfiA

Plasmid

#64901

Purpose

Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1 (273-427)

Plasmid

#162734

Purpose

Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1 domain residues 273-427

Expression

Bacterial

Mutation

Codon optimised

Available Since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2_IRES2_CyOFP1_KCNQ2 var4

Plasmid

#173155

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-CyOFP1

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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