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pCellFree_G06_ZIKV E

Plasmid

#145351

Purpose

Cell-free expression vector for Zika virus Envelope protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Article

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Use

Tags

Expression

Mammalian

Mutation

R1608Q

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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Lenti-DAG1

Plasmid

#205149

Purpose

Overexpress DAG1

Depositor

Monkol Lek

Insert

Dystroglycan 1 (DAG1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Carries common missense variant c.41C>G (p.Ser…

Promoter

EF-1a

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Use

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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LZRS-DNMT1

Plasmid

#24952

Depositor

Paul Khavari

Article

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

DNMT1 (DNMT1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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Tensin-2 WT

Plasmid

#201797

Purpose

Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Use

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Rabenosyn-5

Plasmid

#37538

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_PAX6

Plasmid

#167721

Purpose

Expresses PAX6 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX6 (PAX6 Human)

Use

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_LMTK3

Plasmid

#187773

Purpose

MAC-tagged gene expression of human LMTK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK3 (LMTK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA221rAPOBEC1-T7RNAP

Plasmid

#167979

Purpose

Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

rAPOBEC1-T7RNAP (T7p07 Synthetic)

Use

Tags

T7 tag and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_MYOD1

Plasmid

#167716

Purpose

Expresses MYOD1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYOD1 (MYOD1 Human)

Use

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP-CeNL_pcDNA3

Plasmid

#89514

Purpose

Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLncEXP

Plasmid

#64865

Purpose

Empty vector for lncRNA expression

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
LAMP-ReNL_pcDNA3

Plasmid

#89532

Purpose

Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_N_NFAC3

Plasmid

#167781

Purpose

Expresses NFAC3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

NFAC3 (NFATC3 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_IRF3

Plasmid

#187749

Purpose

MAC-tagged gene expression of human IRF3

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

IRF3 (IRF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTUP1

Plasmid

#104341

Purpose

Express TUP1

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

TUP1 (TUP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab7a-Blasticidin

Plasmid

#120716

Purpose

Expresses late endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab7a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSSN6

Plasmid

#104340

Purpose

Express SSN6

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

SSN6 (CYC8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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CeNL-Clathrin_pEGFP

Plasmid

#89540

Purpose

Cyan color variant of bright luminescent protein, fused to clathrin light chain

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

Clathrin light chain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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