We narrowed to 8,238 results for: p2a

1. MSCV-LMP2A-IRES-mCherry

   Plasmid

   #220353

   Purpose

   Expresses EBV latent gene LMP2A in mammalian cells

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

   Plasmid

   #127872

   Purpose

   Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-mCherry-p2A-eNpHR3.0-TS

   Plasmid

   #158703

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   mCherry-p2A-eNpHR3.0-TS
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

   Plasmid

   #213009

   Purpose

   A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-mKate2-P2A-TurboID-NS

   Plasmid

   #166568

   Purpose

   Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   mKate2-P2A-TurboID
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-SOX2-17-p2a-KLF4

   Plasmid

   #193290

   Purpose

   episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. dSpyCas9-p300-P2A-CASANOVA

   Plasmid

   #113129

   Purpose

   Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   dCas9-p300-P2A-CASANOVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

    Plasmid

    #170237

    Purpose

    Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

    Plasmid

    #230988

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Cre-dependent, membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. mKate2-P2A-APEX2-GBP

    Plasmid

    #67650

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. P2A-mCherry-EhT backbone

    Plasmid

    #215549

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSV40-mCherry-P2A-Hygro

    Plasmid

    #106478

    Purpose

    Expression of mCherry and Hygromycin resistance gene from SV40 promoter

    Depositor

    Alex Hewitt

    Inserts

    mCherry
    Hygromycin B resistance
    

    Use

    Tags

    Expression

    Mammalian and Plant

    Mutation

    Promoter

    SV40 promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-tdTomato-P2A-paCaMKII

    Plasmid

    #165431

    Purpose

    Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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