We narrowed to 18,398 results for: Mut

1. pSG08-pLac-NRII_H139N

   Plasmid

   #102465

   Purpose

   Expresses NRII protein with H139N mutation in E. coli. The expression is controlled by a pLac promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 122606

   Insert

   NRII (ntrB )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Histidine 139 to Asparagine

   Promoter

   pLac

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac Dual PNG172-STII PLU-HIS

   Plasmid

   #112992

   Purpose

   Baculovirus expression of Drosophila png172-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png172 (png Fly)
   plu (plu Fly)
   

   Use

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   png172 mutation

   Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHBS1331 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]

   Plasmid

   #107854

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W385G in splicing reporter

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309S]

   Plasmid

   #107849

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G309S in splicing reporter

   Promoter

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

   Plasmid

   #107847

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All asparagine to glutamine in splicing reporter

   Promoter

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

   Plasmid

   #107843

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All phenylalanine to serine in splicing reporter

   Promoter

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET11a asyn Q79N Q109N (TAC-->TAT)

   Plasmid

   #105738

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Insert

   alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q79N/Q109N

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Flag-Hsp40-S149A/S151A

   Plasmid

   #84998

   Purpose

   Hsp40/DnaJB1 expression plasmid

   Depositor

   Ugo Moens

   Insert

   Hsp40 (DNAJB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Serine 149 and 151 mutated to Alanine

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

   Plasmid

   #73706

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   448-AAAA-451 and D143N

   Promoter

   CMV/tet

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJK289

    Plasmid

    #71553

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-39 to alanine (please see deposito…

    Promoter

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK304

    Plasmid

    #72225

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes alanine-368 to aspartate

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK303

    Plasmid

    #72224

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes alanine-367 to aspartate

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK579

    Plasmid

    #72426

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites

    Depositor

    T. J. Kappock

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    4 silent mutations introduce unique internal SpeI…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGW_SM_BglII

    Plasmid

    #49314

    Purpose

    Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FN 221

    Plasmid

    #50498

    Depositor

    Kenneth Yamada

    Insert

    fibronectin (FN1 Human)
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    repeats 6-10, R1524K, D1526E, C1232A

    Promoter

    T7

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. (-6kb/-207)mtH4-GFP

    Plasmid

    #30464

    Depositor

    Gerhart Ryffel

    Article

    Ryffel et al Mech Dev. 2000 Jan . 90(1):65-75.

    Insert

    HNF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of HNF4 binding site at 5471-5494 (Holew…

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. (-6kb/-58)mtH4-GFP

    Plasmid

    #30463

    Depositor

    Gerhart Ryffel

    Article

    Ryffel et al Mech Dev. 2000 Jan . 90(1):65-75.

    Insert

    HNF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of HNF4 binding site at 5471-5494 (Holew…

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSZBAD44

    Plasmid

    #15337

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein(CTN)
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    F to T mutation

    Promoter

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pSZBAD45

    Plasmid

    #15338

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein(CRN)
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    F to R mutation

    Promoter

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pSZBAD47

    Plasmid

    #15339

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein(CWN)
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    F to W mutation

    Promoter

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only