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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 10381 - 10400 of 16394 results

  3. G protein alpha-o-R179C-EE

    Plasmid
    #55780
    Purpose
    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Bacterial and Mammalian
    Mutation
    Arginine 179 was replaced with cysteine
    Promoter
    CMV
    Available Since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    55780-plasmid-map-sequence-id-122021

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  5. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

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  6. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  10. pEmal10H – MBP-EWS_300-447 (RGG1+RRM)

    Plasmid
    #238369
    Purpose
    Expresses the RGG1-RRM (300-447) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-299, 448-638
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238369-plasmid-map-sequence-id-478743

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  11. T7 Lig-C-HiBiT

    Plasmid
    #215441
    Purpose
    Bacterially expressed T7 Lig-C-HiBit for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Escherichia phage T7 DNA Ligase, C-terminal HiBiT, C-terminal 10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215441-plasmid-map-sequence-id-428723

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Showing: 10381 - 10400 of 16394 results