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pEN-L1-K2-L2

Plasmid

#80684

Purpose

Gateway Entry vector containing a temperature-sensitive mouse DHFR

Depositor

Nico Dissmeyer

Publication

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Inserts

DHFR (Dhfr Mouse)
DHFR (Dhfr Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

mouse DHFR with N-terminal Ubiquitin

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET21c(+)-mtPheRS

Plasmid

#31080

Depositor

Linda Spremulli

Publication

Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

Insert

Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Nt …

Promoter

T7

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
HOXB3 (human) FLAG pSP65

Plasmid

#8523

Depositor

Corey Largman

Publication

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pENTR-SYNZIP18

Plasmid

#80672

Purpose

Encodes the synthetic coiled coil peptide SYNZIP18

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP18

Use

Gateway Cloning

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_CCND1_WT

Plasmid

#82891

Purpose

Gateway Donor vector containing CCND1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CCND1 (CCND1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCWX-R4-DEST-R2-PGR65

Plasmid

#45953

Depositor

Patrick Salmon

Publication

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PEW-pEF1A-inactEGFP

Plasmid

#212009

Purpose

Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

Depositor

Catherine Wu

Publication

Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

Insert

Inactivated eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

pEF1a

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR223-LMP1

Plasmid

#195805

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP1

Depositor

Eric Johannsen

Publication

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP1 (LMP-1 Epstein-Barr virus)

Use

Gateway Cloning

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_C3010

Plasmid

#91098

Purpose

Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

ZmUbi

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_YTHDC1_WT

Plasmid

#81799

Purpose

Gateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YTHDC1 (YTHDC1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RNH1_WT_V5

Plasmid

#82995

Purpose

Gateway Donor vector containing RNH1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RNH1 (RNH1 Human)

Use

Gateway Cloning

Mutation

R188H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFIL3_WT_V5

Plasmid

#82985

Purpose

Gateway Donor vector containing NFIL3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NFIL3 (NFIL3 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCBL101gw

Plasmid

#199725

Purpose

A pVS1-based T-DNA binary vector and destination vector for Gateway cloning with NPTII as a plant selectable marker gene.

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

attR1/R2 cassette

Expression

Plant

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_R250A_ble

Plasmid

#124585

Purpose

Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Publication

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR_R250A (cggR Synthetic)

Use

Synthetic Biology

Mutation

changed arginine 250 to alanine

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOD_C3006

Plasmid

#91096

Purpose

Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

FMV 34S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_MEF2C_WT

Plasmid

#81735

Purpose

Gateway Donor vector containing MEF2C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MEF2C (MEF2C Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pME-nlsGFP-P2A

Plasmid

#80808

Purpose

nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-D-TOPO-PAmCherry1-MCS

Plasmid

#60608

Purpose

Cloning PAmCherry1

Depositor

Steven Chu, Xiaolin Nan

Publication

Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;11

Insert

PAmCherry1

Use

Cloning

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

Plasmid

#172911

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Publication

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ace2N-4AA-mNeon A122D

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPC1000

Plasmid

#174829

Purpose

Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Inserts

CRISPRi SpCas9 sgRNA cassette
PuroR-P2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

EF1a and mouse U6

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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