We narrowed to 20,145 results for: Sele

Showing: 10481 - 10500 of 20145 results

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pcDNA3-nuc-Nb80

Plasmid

#205519

Purpose

Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

Depositor

Jin Zhang

Publication

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-Nb80

Tags

Nuclear localization signal (NLS) and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ALFA-bArr2

Plasmid

#201497

Purpose

Expression of the beta-arrestin2 containing a N-terminal ALFA tag.

Depositor

Patrick Giguère

Publication

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

ALFA-bArr2 (ARRB2 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Publication

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Tags

triple HA tag

Expression

Mammalian

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD9

Plasmid

#195847

Purpose

Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H

Depositor

Gunnar Schulte

Publication

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD9 (FZD9 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-LSSmGFP

Plasmid

#186540

Purpose

Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pmcherry α-catenin L344P

Plasmid

#178647

Purpose

express α-catenin with L344P mutation

Depositor

Daniel Conway

Publication

Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

Insert

α-catenin (CTNNA1 Human)

Tags

mcherry

Expression

Mammalian

Mutation

L344P

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMA4854

Plasmid

#184849

Purpose

Coexpression of PhiC31o recombinase and EGFP

Depositor

Mikhail Alexeyev

Publication

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Inserts

EGFP
PhiC31o

Expression

Mammalian

Promoter

CMV and RSV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKIF1C_R169W-GFP

Plasmid

#185981

Purpose

Mammalian expression of human KIF1C with R169W mutation and GFP tag.

Depositor

Anne Straube

Publication

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Tags

eGFP

Expression

Mammalian

Mutation

R169W - mutation causing HSP (SPG58)

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mito-eqFP650

Plasmid

#174179

Purpose

Express eqFP650 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

eqFP650 with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

Plasmid

#173461

Purpose

C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PIP2-CFP(ST) sensor

Plasmid

#170355

Purpose

CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Publication

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

CFP(ST)-PH (PLCD1 Human)

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

Plasmid

#126697

Purpose

donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Publication

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

TagBFP

Expression

Mammalian

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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H2B-mAvicFP1

Plasmid

#129511

Purpose

Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

H2B-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 fused to the C terminus of human histone…

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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VfAU1-LOV_P2A_casp9

Plasmid

#131224

Purpose

Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV-HA_P2A_myc-casp9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR-Kras

Plasmid

#118408

Purpose

Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-Kras

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Promoter

CMV

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
p CIneo-RL-Let7-3xBulgeB-mut

Plasmid

#115369

Purpose

Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

Depositor

Witold Filipowicz, Ramesh Pillai

Publication

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R

Plasmid

#104548

Purpose

Expresses Cry2-mCherry-PKA W196R in mammalian cells

Depositor

David Lawrence

Publication

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R

Promoter

CMV

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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UNC50-myc

Plasmid

#104382

Purpose

Expresses UNC50-myc wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Publication

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

UNC50 (UNC50 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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