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pcDNA4/TO-2xStrep-mu68

Plasmid

#162627

Purpose

Expresses N-terminal 2x-Strep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL52

Plasmid

#162628

Purpose

Expresses N-terminal 2x-Strep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32

Plasmid

#162629

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGuide-P1-O1a (EL702)

Plasmid

#140040

Purpose

CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

Depositor

Johan Elf

Article

Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

Insert

barcode P1 and sgRNA lacO1 array

Use

Synthetic Biology

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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UL91-2xStrep pCDNA4

Plasmid

#120375

Purpose

Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL91 (UL91 Human Cytomegalovirus (HHV-5))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLR5-U6-sgRNA-EF

Plasmid

#82635

Purpose

piggyBac vector for expressing modified sgRNA in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Type

Empty backbone

Use

CRISPR; Piggybac vector

Expression

Mammalian

Promoter

U6

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 3m shRNA

Plasmid

#122024

Purpose

Negative control shRNA for SEPT11 sh2 shRNA (plasmid 122023).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG030

Plasmid

#113993

Purpose

Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

IPTG-inducible T7RNAP promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG082

Plasmid

#114000

Purpose

Level 0 module. NifH promoter for nodule-specific expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

NifH promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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IDP254

Plasmid

#87004

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postCLAP

Use

Synthetic Biology

Tags

spGFP (1-10)

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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IDP253

Plasmid

#87003

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preCLAP

Use

Synthetic Biology

Tags

GFP11

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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IDP379

Plasmid

#87006

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postLib-mRFP-4xboxB

Use

Synthetic Biology

Tags

mRFP

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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IDP378

Plasmid

#87005

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

preLib-bfp-4xboxB

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-707

Plasmid

#85249

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-709

Plasmid

#85251

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-710

Plasmid

#85252

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-805

Plasmid

#85254

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pH6Z-711

Plasmid

#85253

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-807

Plasmid

#85256

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge