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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,901 results for: cloning

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Showing: 10521 - 10540 of 63901 results

  13. pAG CMV N-HA Puro

    Plasmid
    #237879
    Purpose
    Empty mammalian expression plasmid for cloning N-terminally HA-tagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237879-plasmid-map-sequence-id-471572

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  18. epi-BE4max-NG

    Plasmid
    #135977
    Purpose
    Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Publication
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  19. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  20. pAM5188

    Plasmid
    #120087
    Purpose
    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.
    Depositor
    Susan Golden
    Publication
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    Use
    Other
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120087-plasmid-map-sequence-id-234428

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Showing: 10521 - 10540 of 63901 results