We narrowed to 16,278 results for: nes
-
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
GP803
Plasmid#164547PurposeExpresses Smo-FLAG K434, 444, 448RDepositorInsertSmoothened K434, 444, 448R (Smo Mouse)
UseLentiviralTagsFLAGMutationAll three Lysines in cytoplasmic loop three of Sm…PromoterGgCryD1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164042PurposeCRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-CRISPR-TagV2
UseCRISPRExpressionMammalianAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159848PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159856PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEPLuc-EMCV IRES-Antares
ExpressionMammalianPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)
Plasmid#114100Purposefusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
UseAAVExpressionMammalianPromoterhSynAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Vmn2r1-5Hprt-loxP TV
Plasmid#108079PurposeTergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)DepositorInsertVmn2r1-Hprt (5')-loxP-neo (Vmn2r1 Mouse)
UseMouse TargetingAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE
Plasmid#122056PurposeLentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253PDepositorUseLentiviralExpressionMammalianMutationMyoD chimeric protein substituted with the NeuroD…Available sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-TNFRSF10B
Plasmid#117373PurposeDual luciferase assay for miRNA-targeted UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3' UTR TNFRSF10B
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom0.6
Plasmid#87353PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 0.6 kbpDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter0.6 kbp proximal promoter of human MCT2 (nested d…Available sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-AGMpygTRIM5-FOS
Plasmid#79064PurposeExpress African green monkey pygerythrus TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(African green monkey pygerythrus)-FOS
TagsOneSTrEP-FLAGExpressionMammalianPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3F2
Plasmid#73428PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nuc-Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71349PurposeExpress Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOrange Nano-lantern(Ca2+)_H2B
ExpressionMammalianAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_fibrillarin
Plasmid#65709PurposeExpresses CNL-fibrillarin in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfibrillarin (FBL Human)
UseLuciferaseTagsCNLExpressionMammalianPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_ITPKA
Plasmid#65688PurposeExpresses ITPKA-CNL in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsCNLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_Golgi
Plasmid#65695PurposeExpresses Golgi-ONL in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsONLExpressionMammalianPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPD
Plasmid#64039PurposeMammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
TagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only