We narrowed to 18,028 results for: URE

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binder/tag-WT-FAK-insertionA

Plasmid

#190439

Purpose

The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

FAK (Ptk2 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

SsrA inserted after aa 35

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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bactin:mKate2-ras

Plasmid

#105963

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Ras (KRAS Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pHM130Env

Plasmid

#137665

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DYNLL1

Plasmid

#132530

Purpose

Human LC8-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL1 (DYNLL1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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DYNC2LI1

Plasmid

#132527

Purpose

Human LIC3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNC2LI1 (DYNC2LI1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB1

Plasmid

#132533

Purpose

Human Rbl-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB1 (DYNLRB1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pRSETa-Amrose v1

Plasmid

#79537

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 1

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJ207 Delta-cpc-KanR

Plasmid

#51468

Purpose

Deletes the cpc operon in Synechocystis and confers kanamycin resistance

Depositor

Anastasios Melis

Article

Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

Insert

Replacing the cpc operon with kanamycin resistance

Use

Synthetic Biology

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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XE223 human myc-Chibby-pCS2+MT

Plasmid

#16825

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

myc-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

6-myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pHH0103_PSTPIP1-1/1

Plasmid

#91453

Purpose

Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PSTPIP1-1/1 (PSTPIP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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R9-WT

Plasmid

#125210

Purpose

Expressing wildtype human Rad9b gene

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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RBM6_RRM_pGEX

Plasmid

#135157

Purpose

Recombinant expression of RBM6 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM6 (RBM6 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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Psicheck2 MERTK full length 3'UTR

Plasmid

#26992

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

MERTK Full length 3' UTR (MERTK Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
mKO-Fibrillarin-7

Plasmid

#57842

Purpose

Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Fibrillarin (FBL Human)

Tags

mKO

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_PIK3R1-1/1

Plasmid

#91274

Purpose

Protein expression and purification of human SH3 domain construct PIK3R1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R1-1/1 (PIK3R1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PMYT1_HUMAN_D0

Plasmid

#79687

Purpose

This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PMYT1

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Arp3_pLib

Plasmid

#173677

Purpose

Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp3 (ACTR3 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only