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Plasmid#87292PurposeEncodes Y2H Gal4 activation domain for Hsh155 R294CDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationR294CPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 E291D
Plasmid#87290PurposeEncodes Y2H Gal4 activation domain for Hsh155 E291DDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationE291DPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/K335E
Plasmid#87286PurposeEncodes Hsh155 N295D K335E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295D & K335EPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/D450G
Plasmid#87284PurposeEncodes Hsh155 R294L D450G for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationR294L & D450GPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/K335E
Plasmid#87283PurposeEncodes Hsh155 R294L K335E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationR294L & K335EPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K6C/G76C
Plasmid#86584PurposeBacterial expression of Ubiquitin K6C/G76CDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K6C/G76CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ubiquitin K48C/G76C
Plasmid#86585PurposeBacterial expression of Ubiquitin K48C/G76CDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K48C/G76CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ubiquitin K63C/G76C
Plasmid#86586PurposeBacterial expression of Ubiquitin K63C/G76CDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K63C/G76CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMpGWB233
Plasmid#68624PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(D77R/T214V)
Plasmid#66138PurposeExpresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationD77R, T214VPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SI13-Lrrc3b
Plasmid#46303DepositorInsertLrrc3b mouse monoclonal antibody (low affinity) (lrrc3b Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PTK9L
Plasmid#31587DepositorInsertPTK9L protein tyrosine kinase 9-like (A6-related protein) (TWF2 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH066
Plasmid#42162DepositorInsertN-TAP Tag-RT-::mTFP1
UseTagsTAP-TAgExpressionMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH009
Plasmid#42146DepositorInsertN-TAP-tag::mTFP1
UseTagsTAP-TagExpressionWormMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
ML40K1
Bacterial Strain#61920PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA genes. deleted. Contains pACYC plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
ML12
Bacterial Strain#61910PurposeC43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. ilvE avtA aspC genes deleted.DepositorBacterial ResistanceKanamycinAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-MCS--4x6T-WPRE
Plasmid#196417PurposeEmpty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12a
Plasmid#186446PurposeEncodes Cas12a from F. novicida (type V-A)DepositorInsertCas12a
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk3a2
Plasmid#13759DepositorInsertJnk3a2 (MAPK10 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB120
Plasmid#68574PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b-V106W
Plasmid#215825PurposeExpress CBE6b V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6b V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV SCH9
Plasmid#212708PurposeA SCHEMA evolved AAV variant effective at targeting subventricular NSCsDepositorInsertAAV2 Rep SCH9 Cap
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM189A
Plasmid#218135PurposeFor hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.DepositorInsertSD0
UseTagsrpoZExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6c-V106W
Plasmid#215826PurposeExpress CBE6c V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6c V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Nst-MCS
Plasmid#80487Purposedonor vector for AAVS1 targeting (neomycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGH020_sgRNA_G418-GFP
Plasmid#85405Purposehu6 driven sgRNA vector with G418 and GFP selectable markersDepositorInsertG418 resistance and GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-ASF1A
Plasmid#31591DepositorInsertASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PRB
Plasmid#89130Purposemammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418DepositorInserthuman progesterone receptor-B (PGR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Nst-CAG-DEST
Plasmid#80489PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
loxP-G418-LoxP-TurboID-Rab11 HR
Plasmid#230026PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cellsDepositorInsertgenomic sequence Rab11 locus (RAB11A Human)
UseCRISPRTagsTurbo-ID, V5ExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only