We narrowed to 1,308 results for: LIPI

1. mTagBFP2-FKBP-CYB5Atail

   Plasmid

   #108141

   Purpose

   For recruitment of ER

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mTagBFP2:FKBP1A(3-108):[GGSA]4GG:CYB5A(100-134) (CYB5A, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CYB5A(100-134)

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. LCV2_AAVS1_sgRNA_3

   Plasmid

   #155089

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOET3-6xHis-hSMSr-ΔSAMD

   Plasmid

   #236225

   Purpose

   Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells

   Depositor

   Chiaki Murakami

   Article

   Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

   Insert

   SAMD8 (SAMD8 Human)
   

   Use

   Tags

   Hexa histidine tag (6xHis-tag) and enterokinase s…

   Expression

   Insect

   Mutation

   sterile alpha motif domain at N-terminal (1-231 b…

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

   Plasmid

   #198570

   Purpose

   SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed arginine R76 to alanine (R76A)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-IRES-blast GFP-SPNS1(E164K)

   Plasmid

   #198572

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACE-Truncated-Kir3.2-StreptagII

   Plasmid

   #177263

   Purpose

   Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

   Insert

   G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
   

   Use

   Tags

   StreptagII

   Expression

   Insect

   Mutation

   Residues 49-378

   Promoter

   polyhedrin promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast ACSL3

   Plasmid

   #173165

   Purpose

   Retroviral vector to express sgRNA resistant human ACSL3

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2_mClover3_PSMD1_sgRNA_1

   Plasmid

   #155102

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   PSMD1_sgRNA_1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LCV2_mClover3_AAVS1_sgRNA_4

   Plasmid

   #155101

   Purpose

   lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLCKO_AAVS1_sgRNA_3

    Plasmid

    #155086

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LCV2_mCherry_AAVS1_sgRNA_4

    Plasmid

    #155106

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLCKO_AAVS1_sgRNA_4

    Plasmid

    #155087

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LCV2_AAVS1_sgRNA_4

    Plasmid

    #155090

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBOB-N-Flag-GK (281-473)

    Plasmid

    #134273

    Purpose

    Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk amino acids 281-473

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBOB-N-Flag-GK (12-272)

    Plasmid

    #134272

    Purpose

    Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk amino acids 12-272

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBOB-N-Flag-GK_v1

    Plasmid

    #134270

    Purpose

    Lentivector encoding Flag-tagged GK (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk isoform 1; 524 amino acids

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBP-parallel1-GK5_v2 (D443N)

    Plasmid

    #134293

    Purpose

    Expresses GK5 (isoform 2)-D443N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 443 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMBP-parallel1-GK5_v2 (D280N)

    Plasmid

    #134290

    Purpose

    Expresses GK5 (isoform 2)-D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBP-parallel1-GK5_v2 (D30N)

    Plasmid

    #134289

    Purpose

    Expresses GK5 (isoform 2)-D30N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 30 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA6-C-Flag-GK5_v1

    Plasmid

    #134281

    Purpose

    Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge