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LIPI_pCSdest

Plasmid

#53849

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LIPI (LIPI Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
Mucolipin1-pEGFP C3

Plasmid

#62960

Purpose

full length human Mucolipin-1 cloned into pEGFP C3

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Mucolipin-1 has 1 silent mutation compared to the…

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
Mucolipin1-pHcRed C1

Plasmid

#62959

Purpose

full length human Mucolipin-1 cloned into pHcRed1 C1

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Use

Tags

HcRed1

Expression

Mammalian

Mutation

Mucolipin-1 has 1 silent mutation compared to the…

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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Mucolipin1 D471-472K-pHcRed C1

Plasmid

#62961

Purpose

full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Use

Tags

HcRed

Expression

Mammalian

Mutation

D471/472K (and two silent mutations T77 ACA inst…

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
MSCV FLIPi P2G_Thy1.1 PTEN

Plasmid

#19749

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 p53

Plasmid

#19748

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-puro human lipin 1-1 shRNA

Plasmid

#32019

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
Anti-TRPML3/Mucolipin-3 [N268/19R]

Plasmid

#114477

Purpose

Mammalian Expression Plasmid of anti-TRPML3/Mucolipin-3 (Human). Derived from hybridoma N268/19.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRPML3/Mucolipin-3 (Homo sapiens) recombinant mouse monoclonal antibody (MCOLN3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG wildtype, catalytic active lipin 1

Plasmid

#32005

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 FLAG 17xS/T->A, catalytic active lipin 1

Plasmid

#32007

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

Promoter

CMV, SP6

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG wildtype, catalytic dead lipin 1

Plasmid

#32006

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E and D714E

Promoter

CMV, SP6

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

Plasmid

#32008

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

Promoter

CMV, SP6

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Use

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Use

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-puro FLAG wildtype, catalytic active lipin 1

Plasmid

#32010

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pSUMO FAPP2-GLTPH

Plasmid

#170739

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

PLEKHA8-GLTPH (PLEKHA8 Human)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29-hMIGA2(170-593)-6xhis

Plasmid

#192834

Purpose

Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29-hMIGA2(306-593)-6xhis

Plasmid

#192837

Purpose

Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO GLTP

Plasmid

#170732

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

GLTP (GLTP Human)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge