Addgene

We narrowed to 7,585 results for: Lif;

Showing: 1041 - 1060 of 7585 results

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RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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RIF C-terminal sgRNA

Plasmid

#207096

Purpose

pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AATACTAAATAGAATTTTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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REV7 C-terminal sgRNA

Plasmid

#207095

Purpose

pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GCTCATAAAGGCAGCTGAGG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

His-HRV3C-

Expression

Yeast

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

3X FLAG-HRV3C-

Expression

Yeast

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX-he1.1:YFP

Plasmid

#184816

Purpose

Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDR274-gsc2-sgRNA

Plasmid

#184818

Purpose

Used to synthesize gRNA targeting exon 2 of the gsc2 gene

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

gsc2-sgRNA

Use

CRISPR; Template for sgrna synthesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nmbb

Plasmid

#184822

Purpose

Used to synthesize RNA probe for in situ hybridization for nmbb

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nmbb (nmbb Zebrafish)

Use

Template for synthesis of nmbb probe

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nts

Plasmid

#184823

Purpose

Used to synthesize RNA probe for in situ hybridization for nts

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nts (nts Zebrafish)

Use

Template for synthesis of nts probe

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-mApple-he1.1:CFP

Plasmid

#184814

Purpose

Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-mApple

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-GFP-he1.1:CFP

Plasmid

#184815

Purpose

Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-GFP-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-GFP

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 KO sgRNA

Plasmid

#207103

Purpose

pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGTGTAACGTGGAGCCAGTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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