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pCMV5-ALK2 Q207D

Plasmid

#11740

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Mutation

Q207D: activated receptor

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCMV5B-ALK4

Plasmid

#11756

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK4 (ACVR1B Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
QUAS-5-T7-TetO-GFP_ColE1

Plasmid

#171660

Purpose

QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-5-T7-TetO-GFP ssra-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par6C

Plasmid

#15474

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6C (PARD6A Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5-ALK2 wt

Plasmid

#11741

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pOC1 Halo-Actb KI

Plasmid

#183426

Purpose

CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-p53 gRNA-Cas9-T2A-mCherry

Plasmid

#164256

Purpose

Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sg p53

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1203

Plasmid

#196024

Purpose

Expression/purification of 6xHis-TEV-MlaC

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW FLEx Synapsin-FLAG

Plasmid

#139653

Purpose

Conditional expression of Synapsin-FLAG

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

Synapsin-FLAG

Expression

Mammalian

Promoter

Synapsin

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3-GFP KI

Plasmid

#183432

Purpose

CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5-ALK2 K235R

Plasmid

#11755

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Mutation

K235R: kinase dead

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pVAX-Help-SIINFEKL

Plasmid

#141350

Purpose

DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

HELP-SIINFEKL-KDEL

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo-FKBP KI

Plasmid

#183435

Purpose

CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo-FKBP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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WT TXNIP-FLAG

Plasmid

#200091

Purpose

WT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLOT-syb-sp-YFP1-10

Plasmid

#73700

Purpose

Neuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spYFP1–10 chimera

Use

Lexaop reporter vector

Expression

Insect

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-HA KI

Plasmid

#183427

Purpose

CreON knock-in for GluA1-HA (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo KI

Plasmid

#183449

Purpose

CreOFF knock-in for PSD95-Halo (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 CRISPIE-Halo-Homer1 KI

Plasmid

#183446

Purpose

CreON knock-in for Halo-Homer1 (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and CRISPIE Halo donor

Use

CRISPR

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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