Addgene

We narrowed to 8,650 results for: Ott

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Use

Tags

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-GCAMP6f

Plasmid

#80315

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Insert

GCaMP6f

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RSV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Tags

Expression

Mutation

Promoter

T7

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pQUASp-mCD8GFP

Plasmid

#46163

Purpose

Expression of murine CD8GFP in insects

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

mCD8GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-NS

Plasmid

#108885

Purpose

Multisite gateway vector for 5' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*

Plasmid

#80039

Purpose

Expression of EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pSuper-Sema4A 42-3n

Plasmid

#115173

Purpose

shRNA against rodent Sema4A

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

shRNA targeting Sema4A

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pXJ40-HA-Merlin I S518D

Plasmid

#19701

Depositor

Filippo Giancotti

Article

Okada et al J Cell Biol. 2005 Oct 24. 171(2):361-71.

Insert

Merlin I (Nf2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 518 to Aspartic Acid

Promoter

CMV

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01*-His

Plasmid

#84509

Purpose

E.coli version of EPN01*

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01*-His_pET29b

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-GAL4QF

Plasmid

#61309

Purpose

Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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p6239 pGL4-HPV16-LCR (nt 7000-85)-luciferase

Plasmid

#32889

Depositor

Peter Howley

Article

Ottinger et al Virology. 2009 Dec 20;395(2):161-71.

Insert

LCR HPV16

Use

Tags

Expression

Mutation

Promoter

LCR

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pCMV6 Myc PAK5 K478M

Plasmid

#16020

Depositor

Jonathan Chernoff

Article

Cotteret et al Mol Cell Biol. 2003 Aug . 23(16):5526-39

Insert

PAK5 (PAK5 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K478M: kinase dead

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01

Plasmid

#80046

Purpose

expression of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Use

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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