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Plasmid#219401PurposeInsert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)DepositorInsertEmpty-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)
Plasmid#205045PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L1
Plasmid#205046PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L1
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L2
Plasmid#205047PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L2
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L3
Plasmid#205048PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L3
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L1
Plasmid#205027PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L1
UseTagsKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L2
Plasmid#205028PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L2
UseTagsKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L3
Plasmid#205029PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L3
UseTagsKGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H1
Plasmid#205030PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H1
UseTagsKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2
Plasmid#205031PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2
UseTagsKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H3
Plasmid#205032PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H3
UseTagsKKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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MARS
Plasmid#205232PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
UseTagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIS-krPAM1
Plasmid#25456DepositorInsertPAM1 (Pam Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-3P2A
Plasmid#111772PurposeQuadcistronic construct: Co-express four genes by 3 P2A peptidesDepositorTypeEmpty backboneUseCloning intermediateTagsExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVY-3 sfGFP-tag3
Plasmid#194618Purposefluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coliDepositorInsertsfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGAExpressionBacterialMutationPromoterT7Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
DPP7 (3N0T)
Plasmid#28114PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDPP7:SDC134-D09:C41107 (DPP7 Human)
UseTagsHisExpressionBacterialMutationcontains amino acid residues 28-492PromoterAvailable sinceMarch 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVY-17 TC-TEM1-tag3
Plasmid#194631Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-TEM1-GGSKKRKKRGGSKKRKKRGGSKKRKKR
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterpBADAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-19 TC-sfGFP-tag3
Plasmid#194633Purposefluorescent protein with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-sfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only