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Plasmid#115200PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, cā¦PromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S293D
Plasmid#115201PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, cā¦PromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291D/S293D
Plasmid#115202PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, cā¦PromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-Flag-HA-FKBP-T(WT)
Plasmid#181734Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt taā¦PromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
GreenGate 2.0 Toolkit
Plasmid Kit#1000000241PurposeThe GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.DepositorAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLX1.8CMVMitoTurboRFP
Plasmid#28131DepositorInsertMitoTurboRFP
UseLentiviralTagsCOX VIII signal sequenceExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 C20orf67 WW domain
Plasmid#104273PurposeBacterial expression of WW domain from C20orf67DepositorInsertC20orf67 WW domain (PCIF1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYH78
Plasmid#217386PurposePtrc99A-PAmCherry for bacterial expressionDepositorInsertPAmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA-FLEX
Plasmid#38041DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterCAAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA3
Plasmid#217391PurposepTrc99A-PAmCherry-cI78EP8 for bacterial expressionDepositorInsertcI78EP8
UseTagsPAmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH89
Plasmid#217390PurposePtrc-PAmCherry-mcdBsol for bacterial expressionDepositorInsertmcdBsol
UseTagsPAmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH70
Plasmid#217380PurposepTrc99A-PAmCherry-mcdB for bacterial expressionDepositorInsertmcdB
UseTagsPAmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH76
Plasmid#217384PurposePtrc99A-PAmCherry-L6-PopTag for bacterial expressionDepositorInsertPopTag
UseTagsPAmCherry-Linker6ExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH88
Plasmid#217388PurposePtrc99A-PAmCherry-L78-PopTag for bacterial expressionDepositorInsertPopTag
UseTagsPAmCherry-Linker78ExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX
Plasmid#38042DepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAC111-LST8 (pSW75)
Plasmid#39211DepositorInsertLST8 (LST8 Budding Yeast)
UseTagsExpressionYeastMutationPromoterLST8Available sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorInsertmouse Smoothened cDNA (Smo Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGE-mSmo-YFP
Plasmid#38094DepositorInsertSmo (Smo Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS2 (HIF2a-pRetro-Super)
Plasmid#22100DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralTagsExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targetiā¦PromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS9 (HIF2a-pRetro-Super)
Plasmid#22101DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralTagsExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targetiā¦PromoterAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only