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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,674 results for: Psp

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Showing: 1041 - 1060 of 1674 results

  1. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  3. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  5. GPR63-Tango

    Plasmid
    #66368
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR63 (GPR63 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pLY152

    Plasmid
    #130950
    Purpose
    dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    PpspA-2G6 and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130950-plasmid-map-sequence-id-257362

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  11. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid
    #85181
    Purpose
    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    CA-RIT-NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  13. CA-RIT-NFAT1 (PGK-Puro)

    Plasmid
    #63214
    Purpose
    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    CA-RIT-NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Plasmids to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system (Addgene #1000000080).
    Depositor
    Mikhail Shapiro
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  18. XE2 Xwnt-4

    Plasmid
    #16878
    Depositor
    Randall Moon
    Insert
    Xwnt-4 (wnt4 Frog)
    Use
    Xenopus expression
    Available Since
    March 13, 2008
    Availability
    Academic Institutions and Nonprofits only

  19. pLY1244-T4gp44/62

    Plasmid
    #228205
    Purpose
    Expression in BL21(DE3) of WT T4 clamp loader consisting of gp44 and gp62
    Depositor
    Stephen Benkovic
    Insert
    44/62
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228205-plasmid-map-sequence-id-472133

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  20. XP12 Xbra

    Plasmid
    #16978
    Depositor
    Randall Moon
    Insert
    Xbra (t.S Frog)
    Expression
    Bacterial
    Available Since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
Showing: 1041 - 1060 of 1674 results