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pHH0103_AURKB_Pkinase

Plasmid

#110025

Purpose

Protein expression and purification of AURKB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AURKB_Pkinase (AURKB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Ncam1_1.18.1-AP-His

Plasmid

#71962

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.18.1 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Ncam1_0.0.0-AP-His

Plasmid

#71960

Purpose

Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_0.0.0 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.a-Fc-His

Plasmid

#72180

Purpose

Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.a (Unc5c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_KPNB1_HEAT

Plasmid

#110017

Purpose

Protein expression and purification of KPNB1_HEAT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KPNB1_HEAT (KPNB1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Sema3a(L, del13)-AP-His

Plasmid

#72011

Purpose

Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Sema7a-AP-His

Plasmid

#72049

Purpose

Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema7a (Sema7a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-5

Plasmid

#184017

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR1_Hormone-recep

Plasmid

#109906

Purpose

Protein expression and purification of ESR1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR1_Hormone-recep (ESR1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RPA1_RepA-N

Plasmid

#110032

Purpose

Protein expression and purification of RPA1_RepA-N

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RPA1_RepA-N (RPA1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Sema4c-AP-His

Plasmid

#72030

Purpose

Expresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4c (Sema4c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)

Plasmid

#51369

Depositor

Narry Kim

Article

Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

Insert

Dicer (DICER1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

3' pocket mutant (Y926F, R927A)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
Sema4c-Fc-His

Plasmid

#72156

Purpose

Expresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4c (Sema4c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6b(L)-Fc-His

Plasmid

#72165

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAPK3_Pkinase

Plasmid

#110023

Purpose

Protein expression and purification of MAPK3_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MAPK3_Pkinase (MAPK3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNM2_Dynamin-N

Plasmid

#110008

Purpose

Protein expression and purification of DNM2_Dynamin-N

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNM2_Dynamin-N (DNM2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AP2M1_Adap-comp-sub

Plasmid

#109855

Purpose

Protein expression and purification of AP2M1_Adap-comp-sub

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AP2M1_Adap-comp-sub (AP2M1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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