We narrowed to 6,901 results for: plasmid dna

1. pcDNA5/FRT-HA-rM3D(Gs)

   Plasmid

   #45549

   Purpose

   Expresses rM3D (Gs) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   rM3D (Gs)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-Lin28A

   Plasmid

   #51371

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   Lin28A (LIN28A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-nuc-Nb37

   Plasmid

   #205515

   Purpose

   Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-Nb37
   

   Use

   Tags

   Nuclear localization signal (NLS) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HCMVgH (TB40/E)

   Plasmid

   #136446

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-nuc-Nb80

   Plasmid

   #205519

   Purpose

   Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-Nb80
   

   Use

   Tags

   Nuclear localization signal (NLS) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GoldenGate-VP64

   Plasmid

   #47389

   Purpose

   Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   FLAG, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. DNA-CARzeta-IRESpuro

   Plasmid

   #89345

   Purpose

   2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   DNA-CARzeta
   

   Use

   Lentiviral

   Tags

   SNAPf tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-flag-IARS2-Δ1-48

   Plasmid

   #232341

   Purpose

   Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-48 (indicated as Mito-signal in UniPro…

   Promoter

   CMV

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. miR-722 pCDNA

   Plasmid

   #97162

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   miR-722 (mir722 Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT/TO Plk4-mCherry

    Plasmid

    #80269

    Purpose

    mammalian expression plasmid for Plk4-mCherry

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_3xFlagEzh2 RNA binding mutant

    Plasmid

    #173718

    Purpose

    RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 RNA-binding mutant
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-LAP2-YFP-NLS

    Plasmid

    #25847

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LAP2-YFP-NLS
    

    Use

    Tags

    His and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO/intron/GFP

    Plasmid

    #113547

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT/TO MycLAP-STIL 5A

    Plasmid

    #80267

    Purpose

    mammalian expression plasmid for MycLAP-STIL 5A

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    S1103A, S1108A, S1111A, S1116A, T1119A

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO/intron/FlagCter

    Plasmid

    #113548

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    Flag
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid

    #163080

    Purpose

    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

    Depositor

    Xiaokun Shu

    Insert

    Inactive Mpro mutant C145A and H2B-mIFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mem-TurboID

    Plasmid

    #149409

    Purpose

    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Use

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 NPY pHLeon

    Plasmid

    #235176

    Purpose

    Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

    Depositor

    Kate White

    Article

    Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

    Insert

    NPY-pHLeon (NPY Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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