We narrowed to 45,592 results for: cha;
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Plasmid#98151PurposeExpresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:S495A
TagsHis6-TEVExpressionBacterialMutationChanged serine 495 to alaninePromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:W603A
Plasmid#98153PurposeExpresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:W603A
TagsHis6-TEVExpressionBacterialMutationChanged tryptophan 603 to alaninePromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695Q
Plasmid#98154PurposeExpresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695Q
TagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to glutaminePromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695A
Plasmid#98155PurposeExpresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695A
TagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to alaninePromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:Y696G
Plasmid#98156PurposeExpresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:Y696G
TagsHis6-TEVExpressionBacterialMutationChanged tyrosine 696 to glycinePromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP*
Plasmid#175237PurposeBacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the KmDepositorAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-E2
Plasmid#175243PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to YpetDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
Tags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr β¦ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T-
Plasmid#161497PurposeNon activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7gRNA-smyhc1_977
Plasmid#140867PurposesgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).DepositorInsertzebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Synthetic, Zebrafish)
UseIn vitro transcription of sgrnasAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeT-GFP]
Plasmid#153539PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianPromoterSynAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-Y-L
Plasmid#153546Purposethis is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucineDepositorInserthuman TCR ΞΆ-Chain (CD247 Human)
ExpressionMammalianMutationwith 6 Y in 3 ITAMs mutated to LPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX17-PX (1-111)
Plasmid#119098PurposeBacterial expression of human phox homology (PX) domain, SNX17-PX (1-111)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX16-PX (104-216)
Plasmid#119097PurposeBacterial expression of human phox homology (PX) domain, SNX16-PX (104-216)DepositorInsertSNX16-PX (104-216) (SNX16 Human)
PromoterN/AAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX4-PX (48-186)
Plasmid#119085PurposeBacterial expression of human phox homology (PX) domain, SNX4-PX (48-186)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX32-PX (17-166)
Plasmid#119111PurposeBacterial expression of human phox homology (PX) domain, SNX32-PX (17-166)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX7-PX (89-218)
Plasmid#119088PurposeBacterial expression of human phox homology (PX) domain, SNX7-PX (89-218)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-M11-92
Plasmid#134467PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
ExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only