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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,447 results for: grn

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Showing: 10641 - 10660 of 15447 results

  5. pX330-PITCh-MYO1C

    Plasmid
    #227305
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227305-plasmid-map-sequence-id-452213

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  6. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  7. pX459-sg-mGreenLantern/EGFP

    Plasmid
    #179913
    Purpose
    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.
    Depositor
    Indeever Madireddy, Johan Sosa
    Publication
    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern/EGFP sgRNA
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179913-plasmid-map-sequence-id-358503

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  8. pAT15416-BEAR-mScarlet-target-BFP

    Plasmid
    #162996
    Purpose
    plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker
    Depositor
    Ervin Welker
    Publication
    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting the BEAR-mScarlet plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162996-plasmid-map-sequence-id-322150

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  14. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  18. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232105-plasmid-map-sequence-id-462309

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  19. pBPK1520-SNCA-A53T-ng

    Plasmid
    #181739
    Purpose
    Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach
    Depositor
    Dirk Hockemeyer
    Publication
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Prime editing ngRNA for SNCA-A53T (SNCA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    181739-plasmid-map-sequence-id-356616

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  20. pBPK1520-SNCA-A30P-ng

    Plasmid
    #180018
    Purpose
    Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach
    Depositor
    Dirk Hockemeyer
    Publication
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Prime editing ngRNA for SNCA-A30P (SNCA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180018-plasmid-map-sequence-id-354116

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Showing: 10641 - 10660 of 15447 results