We narrowed to 16,278 results for: nes
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pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA
Plasmid#184549PurposeRetroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tagDepositorUseRetroviralTags3xFLAG and HA tagExpressionMammalianPromoterCMVAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG NBT:NRGIIIa:polyA
Plasmid#193012PurposeTol2 transgenic construct that will express human Neuregulin1 Type IIIa in neuronsDepositorAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NLS-YFP-CCTd
Plasmid#184325PurposeMammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCTd (CACNA1D Human)
ExpressionMammalianAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-3xFlag-Neurog1-attB53
Plasmid#183613PurposeRMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo
Plasmid#179438PurposeExpresses rTetR-KRAB(rat) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-KRAB
ExpressionMammalianPromoterpCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
B3GALT5_pLENTI-CAG-IRES-GFP
Plasmid#177006PurposeMammalian lentiviral expression vector encoding B3GALT5DepositorAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHAF1B_pLENTI-CAG-IRES-GFP
Plasmid#177009PurposeMammalian lentiviral expression vector encoding CHAF1BDepositorAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 R10A R15A-msfGFP
Plasmid#180336Purposemammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEPTIN9_v1 (SEPTIN9 Human)
TagsmsfGFPExpressionMammalianMutationSEPT9_i1 R10A R15APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 NCmut1-msfGFP
Plasmid#180329Purposemammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEPTIN9_v1 (SEPTIN9 Human)
TagsmsfGFPExpressionMammalianMutationSEPT9_i1 R289A R290A K291A K294APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H170
Plasmid#170352PurposemTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-BAP1-G312G-HA
Plasmid#154022PurposeMammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312GDepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationc.936T>G, p.G312G (Synonymous Mutation) by Sit…Available sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
ExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V362F-VSV
Plasmid#139345PurposeExpression of TYK2 V362F protein (rs2304256)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
TagsVSVExpressionMammalianMutationV362FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse 5' Srcap AID GFP Puro
Plasmid#127903PurposeHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap GeneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSrcap (Srcap Mouse, Mustard Weed)
UseDonor plasmidTagsGFP, AID, PuroRAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-D131Y
Plasmid#113424PurposeExpresses hA3A-BE3-D131Y in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_D131YPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRHVPS9
Plasmid#108942PurposeMammalian expression of mRIN2 dRHVPS9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRas and Rab interactor 2 (Rin2 Mouse)
TagsHAExpressionMammalianMutationDeletion of RHVPS9 domain (aa 455-738)PromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mECitrine
Plasmid#105151PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mECitrine
Tags3nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter3nmt1Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
B52 + PIK3R1 sgSTOP
Plasmid#100714PurposeB52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting PIK3R1 (cloned using BbsI)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only
