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SspB-iRFP-DHPH-TIAM1

Plasmid

#176118

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

Plasmid

#162333

Purpose

Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSpCas9-2xVP64

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
amilCP chromoprotein promoter-less

Plasmid

#118008

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA

Plasmid

#220221

Purpose

AAV-STARR-seq validation vector with GFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TC1154

Plasmid

#164876

Purpose

Split GFP reporter plasmid expressing GFP (b11beta

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta11)

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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TC1541A

Plasmid

#164875

Purpose

Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta1-10)

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSKC11 - UTRQ

Plasmid

#110490

Purpose

Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'-UTR (bp 1-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-EGFP

Plasmid

#175827

Purpose

Express EGFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET41a iGluu

Plasmid

#119834

Purpose

Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluu

Tags

GST

Expression

Bacterial

Mutation

S72T

Promoter

T7

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FoxG1

Plasmid

#35547

Depositor

Marius Wernig

Article

Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

FoxG1 (Foxg1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

Plasmid

#192133

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-KY

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (K390A, Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-FOXA1

Plasmid

#219394

Purpose

Express FOXA1 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA1 (FOXA1 Human)

Tags

N terminal Myc tag

Expression

Mammalian

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

Plasmid

#162164

Purpose

Selectable homology donor template for Aag2 cell AGO1

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

RFP
3xFLAG

Use

CRISPR and Cre/Lox; Homology donor template

Tags

AGO1 homology and loxP

Expression

Insect

Promoter

Ae. aegypti PUb and endogenous AGO1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

Plasmid

#153526

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#45

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#45

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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