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pGY225

Plasmid

#239809

Purpose

Express H3-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY232

Plasmid

#239810

Purpose

Express EGFP-P2A-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Tags

2A

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-2p-gfp-halo

Plasmid

#227169

Purpose

For organ-specific expression of GFP-Halo in the pharyngeal muscle of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

GFP

Tags

Halotag

Expression

Worm

Promoter

myo-2

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET29a-α3-E

Plasmid

#187935

Purpose

Express phage alpha3 lysis protein E in bacteria

Depositor

Yi Ma

Article

Ma et al Bioengineering (Basel). 2022 Sep 2;9(9):

Insert

Phage alpha 3 lysis protein E

Tags

Strep II

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HRP-TM

Plasmid

#231497

Purpose

Expressing HRP on the cell surface of mammalian cells

Depositor

Baljit Khakh

Article

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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CAG HRP-TM

Plasmid

#231501

Purpose

Expressing HRP on the cell surface under CAG promoter

Depositor

Baljit Khakh

Article

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Use

AAV

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2

Plasmid

#238982

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Jeffrey Benovic, Vsevolod Gurevich

Article

Sterne-Marr et al J Biol Chem. 1993 Jul 25;268(21):15640-8

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

none

Promoter

SP6

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-cfp29

Plasmid

#233651

Purpose

Mycobacterium tuberculosis Encapsulin CFP29 expression plasmid in E. coli

Depositor

Ye Gao

Article

Berger et al Commun Biol. 2025 Feb 15;8(1):245. doi:

Insert

Encapsulin

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRO286

Plasmid

#233812

Purpose

MoClo-YTK; type2A; Auxin systems; amtRO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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CT751

Plasmid

#233657

Purpose

Dox-inducible VP64-MyoD

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

VP64-MyoD

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT754

Plasmid

#233659

Purpose

FLEx switch with tTA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

tTA FLEx switch

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1b-FLAG

Plasmid

#232144

Purpose

Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ABCB10-eYFP

Plasmid

#206038

Purpose

Express YFP in mitochondria

Depositor

Lufang Zhou

Article

Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

Insert

EYFP

Tags

ABCB10 mitochondrial leading sequence

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAP-U2-1

Plasmid

#236472

Purpose

Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

spH2Ap-3xmCherry spH2Bp-3xGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mRuby2

Plasmid

#233155

Purpose

Retroviral expression of fluorescent mRuby2 reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mRuby2

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRC065d

Plasmid

#225979

Purpose

dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Inserts

dRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS

Use

CRISPR

Promoter

J23107 and J23119

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-EGFPCAAX

Plasmid

#232140

Purpose

Expresses EGFP with a CAAX motif under control of a CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

EGFPCAAX

Use

Zebrafish expression

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ mogat3b-FLAG

Plasmid

#232145

Purpose

Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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