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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 51,521 results for: des.1

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Showing: 10721 - 10740 of 51521 results

  1. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184106-plasmid-map-sequence-id-364015

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  4. pAdeasy-CMV-AE-EF1α-R2

    Plasmid
    #194928
    Purpose
    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis
    Depositor
    Limei Wang
    Article
    Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.
    Inserts
    AE
    EEF1A1 (EEF1A1 Human)
    R2
    Use
    Adenoviral
    Tags
    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c
    Mutation
    a 48 bp linker (Gly3ser)4 was added between the t…
    Promoter
    CMV and EF1a
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    194928-plasmid-map-sequence-id-391107

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  5. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid
    #170196
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…
    Promoter
    CMV51
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170196-plasmid-map-sequence-id-334281

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  6. R620-M01-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.1.7

    Plasmid
    #170188
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…
    Promoter
    CMV51
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170188-plasmid-map-sequence-id-334256

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  8. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  13. pYES2/NTA/Leu-LegA7Δank

    Plasmid
    #216519
    Purpose
    Expression of LegA7 ΔAnk
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 331-454
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216519-plasmid-map-sequence-id-430063

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  14. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid
    #216520
    Purpose
    Expression of LegA7 ΔAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔAnk290
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-454
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216520-plasmid-map-sequence-id-430064

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  15. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid
    #216521
    Purpose
    Expression of LegA7 ΔNAnk264
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk264
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 264-361
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216521-plasmid-map-sequence-id-430065

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  16. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid
    #216522
    Purpose
    Expression of LegA7 ΔNAnk290
    Depositor
    Ralph Isberg
    Insert
    LegA7 ΔNAnk290
    Tags
    HIS-tag
    Expression
    Bacterial and Yeast
    Mutation
    LegA7 missing aa 290-361
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216522-plasmid-map-sequence-id-430066

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  19. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid
    #189562
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189562-plasmid-map-sequence-id-374127

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  20. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 10721 - 10740 of 51521 results