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pIAGO-pegA + aceS

Plasmid

#114457

Purpose

Expression of PegA and AceS in streptomycetes

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

pegA + aceS

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pIAGO-pegA + aceS

Plasmid

#114457

Purpose

Expression of PegA and AceS in streptomycetes

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

pegA + aceS

Expression

Bacterial

Promoter

ermE

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSG07-pLac-NRII_L16R

Plasmid

#102464

Purpose

Expresses NRII protein with L16R mutation in E. coli. The expression is controlled by a pLac promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 122606

Insert

NRII (ntrB )

Expression

Bacterial

Mutation

Leucine 16 to Arginine

Promoter

pLac

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSG07-pLac-NRII_L16R

Plasmid

#102464

Purpose

Expresses NRII protein with L16R mutation in E. coli. The expression is controlled by a pLac promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 122606

Insert

NRII (ntrB )

Expression

Bacterial

Mutation

Leucine 16 to Arginine

Promoter

pLac

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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x.Setd7

Plasmid

#87133

Purpose

mammalian expression of Setd7

Depositor

Francesca Spagnoli

Article

Kofent et al Development. 2016 Oct 1;143(19):3573-358

Insert

Setd7 (setd7.L Frog)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only
x.Setd7

Plasmid

#87133

Purpose

mammalian expression of Setd7

Depositor

Francesca Spagnoli

Article

Kofent et al Development. 2016 Oct 1;143(19):3573-358

Insert

Setd7 (setd7.L Frog)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only
pJK520

Plasmid

#72479

Purpose

Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene

Depositor

T. J. Kappock

Inserts

formylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon

Use

Gene synthesis

Tags

His6

Mutation

synthetic gene

Promoter

n/a and none

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK520

Plasmid

#72479

Purpose

Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene

Depositor

T. J. Kappock

Inserts

formylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon

Use

Gene synthesis

Tags

His6

Mutation

synthetic gene

Promoter

n/a and none

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK559

Plasmid

#71603

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK562

Plasmid

#71604

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK604

Plasmid

#71610

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK664

Plasmid

#71613

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tyrosine-154 …

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK666

Plasmid

#71615

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tryptophan-97…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK563

Plasmid

#71689

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK559

Plasmid

#71603

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK562

Plasmid

#71604

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK604

Plasmid

#71610

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK664

Plasmid

#71613

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tyrosine-154 …

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK666

Plasmid

#71615

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tryptophan-97…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK563

Plasmid

#71689

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge