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pWOL-YFP-GW

Plasmid

#209365

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pME-NLS-tdMCP-eGFP

Plasmid

#86243

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP-eGFP

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMM597

Plasmid

#68886

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pESC-HIS (V5 and HA)

Plasmid

#222487

Purpose

Co-expression of two genes in yeast with V5 and HA tags and HIS3 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMH94

Plasmid

#91720

Purpose

Integration-proficient vector for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin

Depositor

Graham Hatfull

Article

Lee et al Proc Natl Acad Sci U S A. 1991 Apr 15;88

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 GW delCMV

Plasmid

#29496

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Expression

Mammalian

Available Since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CRISPR-Puro

Plasmid

#208646

Purpose

pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

Depositor

Quanfu Ma

Type

Empty backbone

Use

CRISPR

Tags

Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

Expression

Mammalian

Promoter

CMV and U6 in tandem

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ134A

Plasmid

#69276

Purpose

Golden Gate entry vector; 4th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDmDERusk

Plasmid

#213702

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pEERM4 Cm

Plasmid

#64026

Purpose

Plasmid for integration into neutral site 2 of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-CPDSalI

Plasmid

#38252

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pEEK2

Plasmid

#83492

Purpose

Expression plasmid for conjugational transfer into cyanobacteria

Depositor

Pia Lindberg

Article

Miao et al Metab Eng Commun. 2017 Jul 29;5:45-53. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Strep-tag

Expression

Bacterial

Promoter

Ptrc core

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-mRFP

Plasmid

#36972

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pMKV060

Plasmid

#133315

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+

Expression

Plant

Promoter

AtUbi10

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEERM3 Km

Plasmid

#64025

Purpose

Plasmid for integration into neutral site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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PB-U6insert

Plasmid

#104536

Purpose

A piggybac vector with a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUM

Plasmid

#64176

Purpose

harbours marker genes URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-LEU2

Plasmid

#122909

Purpose

LYS2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pST1374

Plasmid

#13426

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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