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We narrowed to 28,641 results for: tat

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Showing: 10761 - 10780 of 28641 results

  1. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  9. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid
    #206122
    Purpose
    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    Cav2.1-P1217fs, R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206122-plasmid-map-sequence-id-415911

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  10. Venus-Bik-4E-pEGFP-C1

    Plasmid
    #166744
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Bik-4E (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166744-plasmid-map-sequence-id-328837

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  12. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105982
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    FAPdL5-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Promoter
    human synapsin 1
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105982-plasmid-map-sequence-id-235274

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Showing: 10761 - 10780 of 28641 results