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NSP2-Flag

Plasmid

#210344

Purpose

Expresses SARS-CoV-2 NSP2 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGCC2-FLAG

Plasmid

#214166

Purpose

Transient expression of GCC2

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

GCC2 (Gcc2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

Plasmid

#207050

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

P85L P86L

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

Plasmid

#207051

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

T56A R65A T87A

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

Plasmid

#207052

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85A P86A

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

Plasmid

#207053

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85L P86L

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Blast

Plasmid

#214014

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Hygro

Plasmid

#214015

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Blast-TNNT2-Zeo

Plasmid

#214018

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-mCherry

Plasmid

#210377

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3C (ORF1ab SARS-CoV-2)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-mXRCC1pd-eGFP

Plasmid

#206035

Purpose

Mammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

mXRCC1pd

Tags

eGFP

Expression

Mammalian

Mutation

S105A, S186A, K188A, S195A, S221A, S222A,S238A, S…

Promoter

Ef1a

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_5H_eGFP

Plasmid

#208861

Purpose

Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 5 helix bundle (TLNRD1 Human)

Expression

Mammalian

Mutation

Deleted amino acid 142-273

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003(K31TAG)-Talin rod-mScarletI

Plasmid

#210022

Purpose

Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI

Tags

C-terminal mScarletI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SEC61B-2Strep

Plasmid

#210375

Purpose

Expresses SEC61B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SEC61B (SEC61B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM106B-2Strep

Plasmid

#210360

Purpose

Expresses TMEM106B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 genomic A. thaliana PLDGAMMA3

Plasmid

#209188

Purpose

pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT4G11840 (PLDGAMMA3 Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

Plasmid

#198381

Purpose

Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

UAS:sypb-egfp-cry2

Use

Tol2

Tags

EGFP

Mutation

D387A

Promoter

UAS

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-b(E184Q)-HA

Plasmid

#209264

Purpose

Mammalian expression of ATP11C isoform b mutant E184Q

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Use

Retroviral

Tags

HA

Mutation

E184Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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