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pFGL1344

Plasmid

#116902

Purpose

Alp6:Bar:mCherry (SPB/MTOC marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Alp6 (partial ORF)
Alp6 downstream region

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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-226bp goosecoid luciferase

Plasmid

#17158

Depositor

Ken Cho

Article

Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Insert

goosecoid promoter (gsc-a Frog)

Use

Luciferase

Tags

luciferase

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pRmHa3-dKDM4A-HA2FL2

Plasmid

#20232

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG2 and HA2

Expression

Insect

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A

Plasmid

#20233

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
U7 Promotor-Terminator

Plasmid

#254838

Purpose

Guide-targeted RNA editing mediated by U7 snRNA

Depositor

Eugene Yeo

Article

Smargon et al Nat Chem Biol. 2025 Sep 18:10.1038/s4158

Insert

U7 snRNA Promoter-Terminator

Expression

Bacterial

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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U6 Promoter-Terminator

Plasmid

#254837

Purpose

Guide-targeted RNA editing mediated by U6 snRNA

Depositor

Eugene Yeo

Article

Smargon et al Nat Chem Biol. 2025 Sep 18:10.1038/s4158

Insert

U6 snRNA Promoter-Terminator

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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U1 promoter-terminator

Plasmid

#254836

Purpose

Guide-targeted RNA editing mediated by U1 snRNA

Depositor

Eugene Yeo

Article

Smargon et al Nat Chem Biol. 2025 Sep 18:10.1038/s4158

Insert

U1 snRNA Promoter-Terminator

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD_AIcrVIA3

Plasmid

#234054

Purpose

Plasmid for AIcrVIA3 expression during phage assay

Depositor

Gavin Knott

Article

Taveneau et al Nat Chem Biol. 2026 Jan 26. doi: 10.1038

Insert

AIcrVIA3

Tags

6XHis

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD_AIcrVIA2

Plasmid

#234053

Purpose

Plasmid for AIcrVIA2 expression during phage assay

Depositor

Gavin Knott

Article

Taveneau et al Nat Chem Biol. 2026 Jan 26. doi: 10.1038

Insert

AIcrVIA2

Tags

6XHis

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD_AIcrVIA1

Plasmid

#234052

Purpose

Plasmid for AIcrVIA1 expression during phage assay

Depositor

Gavin Knott

Article

Taveneau et al Nat Chem Biol. 2026 Jan 26. doi: 10.1038

Insert

AIcrVIA1

Tags

6XHis

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pSMT3-fecB

Plasmid

#240158

Purpose

expresses FecB of M. marinum in mycobacteria

Depositor

Wilbert Bitter

Article

Lissner et al mBio. 2025 Dec 12:e0211925. doi: 10.1128

Insert

fecB (MMAR_1650)

Expression

Bacterial

Promoter

HSP60

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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RcP9_AraC_S170_LOV_P190_Sil_90bp

Plasmid

#242158

Purpose

Recombineering Plasmid 9. Positive control to test for successful editing using AraC S170 LOV (silent mutation of P190, 90bp retron)

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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L3928 Filler DNA

Plasmid

#244193

Purpose

Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN1>Fas-Myc/Kir2.1*(E224G Y242F):P2A :dTomato

Plasmid

#242233

Purpose

Express Kir 2.1 and dTomato in the neurons

Depositor

Qingchun Tong

Article

Li et al Cell Rep. 2025 Jun 19;44(7):115863. doi:

Inserts

Fas
Myc/Kir2.1 (E224GY242F)

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

SYN

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--Pconst-YFP

Plasmid

#228099

Purpose

recA promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--Pconst-YFP

Plasmid

#228100

Purpose

constitutive promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PrecA-YFP

Plasmid

#228101

Purpose

constitutive promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PgadX-YFP

Plasmid

#228102

Purpose

constitutive promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--PrecA-YFP

Plasmid

#228094

Purpose

gadX promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--PgadX-YFP

Plasmid

#228095

Purpose

gadX promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--Pconst-YFP

Plasmid

#228096

Purpose

gadX promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--PgadX-YFP

Plasmid

#228098

Purpose

recA promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGL2-deltaTAR-luciferase

Plasmid

#215896

Purpose

Expression of luciferase

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

luciferase

Use

Luciferase

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-CERS5

Plasmid

#166158

Purpose

Gateway entry vector for N. furzeri CERS5

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

CERS5

Use

Gateway Cloning

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-FL

Plasmid

#215839

Purpose

Expression of FLAG/HA tagged firefly luciferase (FL)

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

FLAG/HA tagged firefly luciferase (FL)

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL661-WT(TTTT)

Plasmid

#215864

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL661 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL69-WT(TTTT)

Plasmid

#215847

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL864R-WT(TTTT)

Plasmid

#215860

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL864R with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL661-TTTA

Plasmid

#215865

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL661 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-EGFP12-TTTA

Plasmid

#215857

Purpose

This plasmid encodes sgRNA that target EGFP with stretch sequence

Depositor

Kumiko Ui-Tei

Article

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-EGFP12 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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