Addgene

We narrowed to 2,092 results for: SAG

Showing: 1061 - 1080 of 2092 results

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

Plasmid

#158952

Purpose

Express human RAD51(F129A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed phenylalanine 129 to alanine

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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SMC2-mAC donor (Hygro)

Plasmid

#140648

Purpose

SMC2 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

Plasmid

#140533

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK321 (TOP2A-mAC (Neo))

Plasmid

#140652

Purpose

TOP2A tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

Plasmid

#140535

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK322 (TOP2A-mAC (Hygro))

Plasmid

#140653

Purpose

TOP2A tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLVTHM-shRnd3

Plasmid

#86437

Purpose

2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

Depositor

Violaine Moreau

Article

Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

Insert

shRNA targeting RND3 (RND3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin1

Plasmid

#108888

Purpose

Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin1 (altered codon usage) (CAVIN1 Synthetic, Human)

Use

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-221-PGK-GFP-Blast

Plasmid

#31519

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pSK+ mBMPR-1B DN

Plasmid

#49530

Purpose

cloning vector for dominant negative mouse BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (Bmpr1b Mouse)

Use

Tags

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also L2V

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pCI-Neo-PTPRT (R632X)

Plasmid

#16629

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT R632X (PTPRT Human)

Use

Tags

Expression

Mammalian

Mutation

truncated R632X mutant

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
LRG-2.1T-neo-sgTAF12(human)#4.4

Plasmid

#105590

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12 HFD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pME-H2B

Plasmid

#108883

Purpose

Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)

Use

Multisite gateway

Tags

Expression

Mutation

human amino acid sequence but different codon usa…

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)

Plasmid

#15385

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS123…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
MSCV-PIG-FLAG-TAF4(827-909)

Plasmid

#105617

Purpose

retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBacPak-HA-Non-stop

Plasmid

#52289

Purpose

Expresses Drosophila Non-stop

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Non-stop (not Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
MDH1-miR-221SM-PGK-GFP-Blast

Plasmid

#31520

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196a-1SM-PGK-GFP-Blast

Plasmid

#31510

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196a1 (Mir196a-1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
Hoxa4-3'UTR

Plasmid

#31524

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxa4-3'UTR (Hoxa4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only