We narrowed to 7,632 results for: ef1a

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pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

Plasmid

#193752

Purpose

human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAF0286 Ef1a-Cp36_attB-BFP acceptor

Plasmid

#193474

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

Plasmid

#171027

Purpose

Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1 and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

ChrimsonR is fused to tdTomato

Promoter

Ef1a

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH

Plasmid

#235294

Purpose

ComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6F

Plasmid

#137124

Purpose

Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-ASAP3-WPRE

Plasmid

#132318

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-SunTag-RPS7-PP7-WPRE

Plasmid

#198338

Purpose

PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

GCN4x24-RPS7-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops and SunTag

Expression

Mammalian

Promoter

Ef1a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-N2c_envA-IRES-Neo

Plasmid

#172368

Purpose

Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

envA + Neo

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-WPRE

Plasmid

#218745

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

Ef1a

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

Plasmid

#179459

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV1

Insert

JEDI-2P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(K161N)-A92mKO2

Plasmid

#213554

Purpose

Lentiviral vector for stable expression of K161N Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

K161N mutation in Parkin

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin(S65A)-A92mKO2

Plasmid

#213557

Purpose

Lentiviral vector for stable expression of S65A Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S65A mutation in Parkin

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

Plasmid

#35498

Purpose

AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-GCaMP6s-WPRE-pGHpA

Plasmid

#67526

Purpose

"Fluorescent reporter for calcium imaging"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

GCaMP6s

Use

AAV

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Ngn3ER-PuroR-mCherry

Plasmid

#197385

Purpose

To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

Ngn3ER-2A-PuroR-2A-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald D40G

Plasmid

#164211

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

D40G

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-stGtACR2-FusionRed

Plasmid

#217975

Purpose

AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

Depositor

Edward Callaway

Article

Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

Insert

stGtACR2-FusionRed

Use

AAV

Tags

FusionRed

Promoter

EF1a

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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