We narrowed to 1,219 results for: proc.2

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pcDNA5/FRT/TO - RBM28 E8 G>T minigene

Plasmid

#169272

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 G>T minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(946G>T)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

Plasmid

#169274

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 Exon1-6 GT>A minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(541+1_541+2delinsA)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MAC-CTSL

Plasmid

#172414

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Procathepsin L (Cathepsin L1) (CTSL Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CRISPRi sgSOS1-2

Plasmid

#253093

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Publication

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#2

Plasmid

#189988

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Publication

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Fusion of Zika NS2B-NS3 proteins

Plasmid

#204815

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS2B-NS3 fusion (orf1ab Zika)

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCK-Drosha(UniProt)-FLAG-mEGFP

Plasmid

#164691

Purpose

Express Drosha-FLAG-mEGFP in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Tags

FLAG and mEGFP

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir181b-1-CMV2-mir1

Plasmid

#164670

Purpose

Express miR-181b-1 and miR-1-1 in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-181b-1 (MIR181B1 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon taCasp3-TEVp

Plasmid

#256276

Purpose

Expresses autocleavable Caspase3 in Cre and Flp expressing cells

Depositor

Jean-François Poulin

Publication

Bolduc et al Proc Natl Acad Sci U S A. 2026 Sep;123(3

Insert

INTRSECT taCasp3-TEVp

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Mutation

None

Promoter

nEF

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon taCasp3-TEVp

Plasmid

#256275

Purpose

Expresses autocleavable Caspase3 in Flp expressing and Cre non-expressing cells

Depositor

Jean-François Poulin

Publication

Bolduc et al Proc Natl Acad Sci U S A. 2026 Sep;123(3

Insert

INTRSECT taCasp3-TEVp

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Mutation

None

Promoter

nEF

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff taCasp3-TEVp

Plasmid

#256274

Purpose

Expresses autocleavable Caspase3 in Cre expressing and Flp non-expressing cells

Depositor

Jean-François Poulin

Publication

Bolduc et al Proc Natl Acad Sci U S A. 2026 Sep;123(3

Insert

INTRSECT taCasp3-TEVp

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Mutation

None

Promoter

nEF

Available since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pNoTag-DGCR8

Plasmid

#164694

Purpose

Express DGCR8 without tag in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

DGCR8 (DGCR8 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

Plasmid

#203841

Purpose

non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

Depositor

Ivo Spiegel

Publication

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has service

AAV2

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Mutation

G144A, Y145A, G146A

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir125-CMV2-mir1

Plasmid

#164668

Purpose

Express miR-125a and miR-1-1 in mammalian cells

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-125a (MIR125A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Publication

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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