We narrowed to 6,574 results for: val
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Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR KIF17 (1-738)
Plasmid#60406PurposeExpression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738DepositorInsertKIF17 (KIF17 Human)
UseLentiviralTagsGB1, Strep-Tag, and sfGFPExpressionMammalianMutationTruncated version amino acids 1-738PromoterSFFVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)
Plasmid#78516PurposeLentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)DepositorInsertLAT (LAT Human)
UseLentiviralTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX-Frb:Akt2-IRES-Hygromycin
Plasmid#120713PurposeExpresses Frb:Akt2 fusion protein in mammalian cells & for virus productionDepositorInsertAKT2 (AKT2 Human)
UseLentiviralTagsFRB(T2098L)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1
Plasmid#45822DepositorInsertAS9(Mito MTS)-Delta N67-mMCL1
UseTagsExpressionMammalianMutationATP Synthase subunit 9 is used as a Mito Targetin…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G
Plasmid#169315PurposeDoxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent proteinDepositorInsertmiR-125b-2 (MIR125B2 Human)
UseLentiviralTagsdTomatoExpressionMutationPromoterTREAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PHR-LAT Y6F YFFF-mCitrine (XSB589)
Plasmid#78517PurposeLentiviral vector for expressing LAT-Y6F YFFF-mCitrine (0 Grb2 binding sites)DepositorInsertLAT (LAT Human)
UseLentiviralTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-LAT-Y6F YYYY-mCitrine (XSB586)
Plasmid#78515PurposeLentiviral vector for expressing LAT-Y6F YYYY-mCitrine (3 Grb2 binding sites)DepositorInsertLAT (LAT Human)
UseLentiviralTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3_5UTR_Myc_FLuc RBM42 deletion
Plasmid#229503PurposeMyc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.DepositorInsertMyc 5'UTR_Del 363
UseTagsExpressionMammalianMutationDeletion of bp 363-394PromoterSV40Available sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_5UTR_Myc_FLuc Compensatory Mutant
Plasmid#229504PurposeMyc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42DepositorInsertMyc 5'UTR_CompMutant
UseTagsExpressionMammalianMutationMutations in bp 363-394PromoterSV40Available sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.mTagBFP2.miR-125b-2
Plasmid#169311PurposeConstitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent proteinDepositorInsertmiR-125b-2 (MIR125B2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail delta K
Plasmid#60404PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPKDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C
Plasmid#60398PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C
Plasmid#60405PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
UseTagsExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only