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pSLIK-Hygro-Myc-hAgo2

Plasmid

#60234

Purpose

Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

Insert

Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pAG27

Plasmid

#226742

Purpose

Expresses a reporter for a mitochondrial matrix protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

COX4 (COX4I1 Human)

Use

Lentiviral

Tags

GFP1-10

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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VPS13C^halo∆1235-1748

Plasmid

#187297

Purpose

Express VPS13C truncation mutant in mammalian cells

Depositor

Pietro De Camilli

Article

Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS13C truncation mutant (VPS13C Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCitrine-GFP11

Plasmid

#61667

Purpose

Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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AA145

Plasmid

#215937

Purpose

Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NES-PAmCherry-cPHx3

Plasmid

#116861

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

PAmCherry

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-C1-FKBP-CYB5tail

Plasmid

#202686

Purpose

rapamycin-induced recruitment of proteins to ER

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Igfbp1-promoter/pGL3

Plasmid

#12146

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

IGFBP1 promoter (Igfbp1 Rat)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains the canonical insulin response sequence …

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3s-ABE7.10

Plasmid

#113128

Purpose

expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE 7.10

Tags

HA epitope and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pINT-PuroDDHNF4a2

Plasmid

#31443

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

HNF4A (HNF4A Human)

Use

Phic31/attb

Tags

FKBP L106P

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 DLC1

Plasmid

#24266

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1, DLC1 Human)

Tags

T7

Expression

Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His-mEos3.2

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMMDS DEST

Plasmid

#206264

Purpose

DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Cre-donor, multimate/gateway dest vector (multiba…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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