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pKM342

Plasmid

#71486

Purpose

Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-loxP cassette

Use

Source of loxp-hyg-loxp for pcr amplification.

Expression

Bacterial

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pKM342

Plasmid

#71486

Purpose

Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-loxP cassette

Use

Source of loxp-hyg-loxp for pcr amplification.

Expression

Bacterial

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFTP1

Plasmid

#64952

Purpose

source of TpR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-dhfrII-FRT

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pFTP1

Plasmid

#64952

Purpose

source of TpR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-dhfrII-FRT

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDB4279

Plasmid

#98698

Purpose

A control plasmid containing the intact bsdMX marker

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDB4279

Plasmid

#98698

Purpose

A control plasmid containing the intact bsdMX marker

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXP741

Plasmid

#46059

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP741

Plasmid

#46059

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pMS2-1

Plasmid

#220627

Purpose

Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMS2-1

Plasmid

#220627

Purpose

Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMT-P4

Plasmid

#120866

Purpose

Encodes permuteposon P4 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P4

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-P4

Plasmid

#120866

Purpose

Encodes permuteposon P4 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P4

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMMK801

Plasmid

#207140

Purpose

Type 8 Tn10 backbone part, with RFP dropout

Depositor

Nicholas Shikuma

Article

Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

Type

Empty backbone

Promoter

n/a

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK801

Plasmid

#207140

Purpose

Type 8 Tn10 backbone part, with RFP dropout

Depositor

Nicholas Shikuma

Article

Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

Type

Empty backbone

Promoter

n/a

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-P2

Plasmid

#120864

Purpose

Encodes permuteposon P2 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P2

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-P2

Plasmid

#120864

Purpose

Encodes permuteposon P2 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P2

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBS35

Plasmid

#83797

Purpose

Useful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

mCherry

Tags

mCherry

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBS35

Plasmid

#83797

Purpose

Useful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

mCherry

Tags

mCherry

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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