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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3

Plasmid

#58458

Purpose

Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Publication

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pTK157_mCherry-4.1G-CTD

Plasmid

#46359

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-C

Plasmid

#41477

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-T

Plasmid

#41479

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
TAL2324

Plasmid

#36750

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Left (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2325

Plasmid

#36751

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Right (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2342

Plasmid

#36762

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTCH1-TALEN_Left (PTCH1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (T56D)

Plasmid

#24532

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56D) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74A)

Plasmid

#24258

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87A) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Alanine

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S58A)

Plasmid

#24249

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S58A) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
M19 pCS-twhh-dsl-1myc

Plasmid

#17156

Depositor

Randall Moon

Publication

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

dorsalin-1 (GDF2 Chicken)

Tags

myc-tag and twhh promoter (D rerio)

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFbx15(-544/-527)-TK-Luc

Plasmid

#13414

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf159

Plasmid

#12847

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Publication

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf159

Expression

Mammalian

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Publication

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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SKM2 X057_pECIA14

Plasmid

#114914

Purpose

Prey vector SKM2 X057_pECIA14 should be used with bait vector SKM2 X057_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G56040 (AT5G56040 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SKM1 nA12_pECIA14

Plasmid

#114822

Purpose

Prey vector SKM1 nA12_pECIA14 should be used with bait vector SKM1 nA12_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G25790 (AT2G25790 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA14

Plasmid

#114768

Purpose

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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