We narrowed to 24,056 results for: BASE

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nSaCas9 M732 miniABEMax V82G

Plasmid

#135382

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M732 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 M741 miniABEMax V82G

Plasmid

#135383

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M741 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 N120 miniABEMax V82G

Plasmid

#135384

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 N120 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 ABEMax

Plasmid

#135356

Purpose

Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-ABEMax aa 1058

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 V82G ABEMax

Plasmid

#135361

Purpose

Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1058 nSpyCas9 V82G ABEMax

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE3

Plasmid

#120397

Purpose

Expresses HF2-BE3 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE3

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-Gb2-K

Plasmid

#25759

Purpose

Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pScaf-3024.1

Plasmid

#111404

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-3024

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMi(3xP3-DsRed)

Plasmid

#102539

Purpose

Transgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed marker

Depositor

Michalis Averof

Article

Pavlopoulos et al Proc Natl Acad Sci U S A. 2005 May 31;10

Insert

Minos transpoable element with 3xP3-DsRed marker

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pScaf-3024.1

Plasmid

#111404

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-3024

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMi(3xP3-DsRed)

Plasmid

#102539

Purpose

Transgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed marker

Depositor

Michalis Averof

Article

Pavlopoulos et al Proc Natl Acad Sci U S A. 2005 May 31;10

Insert

Minos transpoable element with 3xP3-DsRed marker

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 N

Plasmid

#189651

Purpose

rbc L 294 Right_G1397 N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJRK258

Plasmid

#173751

Purpose

fluorescent par-6(WT) expression

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::mKate2::par-6::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK259

Plasmid

#173752

Purpose

fluorescent par-6(delPB1) expression

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR

Expression

Worm

Mutation

delPB1

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2039_N2only

Plasmid

#140682

Purpose

Control (“C”) lacking sg-3

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-3

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2040_N2only

Plasmid

#140683

Purpose

Control (“C”) lacking sg-4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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