We narrowed to 15,982 results for: LEA

1. pCX-H2B-KikGR

   Plasmid

   #98649

   Purpose

   Expresses a nuclear-localized photoconvertible green-to-red fluorescent protein

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

   Insert

   KikGR
   

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   CMV-IE enhancer chick beta actin promoter

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pBad-UGAcS

   Plasmid

   #172137

   Purpose

   bacteria expression plasmid for a UDP-GlcNAc sensor

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

   Insert

   UGAcS
   

   Expression

   Bacterial

   Mutation

   H605Q- please see depositor comments

   Promoter

   araBAD

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETZZtevPP7CP(C68A,C73A)

   Plasmid

   #27548

   Depositor

   Kathleen Collins

   Article

   Hogg et al RNA. 2007 Jun . 13(6):868-80.

   Insert

   Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
   

   Tags

   TEV protease cleavage site, six histidine tag, an…

   Expression

   Bacterial

   Mutation

   changed two cysteine to alanine (C68A,C73A) to in…

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pelBHisTEV

   Plasmid

   #157741

   Purpose

   empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Tags

   PelB signal sequence + His10-tag + TEV protease c…

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-TarHom-Tol2-kanaR-cryaa-CFP

   Plasmid

   #74151

   Purpose

   BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

   Depositor

   Holger Knaut

   Article

   Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

   Insert

   Tol2-kanaR-cryaa-CFP
   

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-mCherry

   Plasmid

   #108880

   Purpose

   Expression of human histone2B fused to mCherry (nuclear marker)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   H2B-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Expression

   Mammalian

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-StagRFP

   Plasmid

   #173014

   Purpose

   Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   StagRFP
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS253

   Plasmid

   #114271

   Purpose

   SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

   Insert

   Cbr-Unc-119
   

   Use

   CRISPR and Cre/Lox

   Mutation

   *please see depositor comment below

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVTU_260_BirA

   Plasmid

   #63682

   Purpose

   Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

   Insert

   BirA
   

   Tags

   6X Histidine and TEV cleavage site

   Expression

   Yeast

   Promoter

   ADH1/yeast

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HECTD1

    Plasmid

    #32860

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECTD1 (HECTD1 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 1879-1966 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. AviTag-SpyCatcher

    Plasmid

    #72326

    Purpose

    Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    AviTag-SpyCatcher
    

    Tags

    N-term His6 tag and TEV cleavage site

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. BRD7 (BRD7A-c005)

    Plasmid

    #98245

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD7A (BRD7 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00439:Bromodomain 136-223

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST14-DoubleCatcher-H-Lock

    Plasmid

    #216285

    Purpose

    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher H-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5UAS zfSynaptophysin:GFP

    Plasmid

    #74316

    Purpose

    A fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeats

    Depositor

    Martin Meyer, Stephen Smith

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Insert

    synaptophysin
    

    Tags

    EGFP

    Mutation

    Q12H and deletion of F13 (please see depositor co…

    Promoter

    5UAS

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-SnoopTag-AffiHER2-SpyTag

    Plasmid

    #216280

    Purpose

    Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-AffiHER2-SpyTag
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZY101

    Plasmid

    #73932

    Purpose

    This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html

    Depositor

    Zhanyuan Zhang

    Article

    Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

    Insert

    bar
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. UAS-Syt::Halo7

    Plasmid

    #87648

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::Halo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::Halo7
    

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FnR9-10

    Plasmid

    #182567

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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