We narrowed to 15,822 results for: LEA

1. pCDNA-FRT-FAK20

   Plasmid

   #59120

   Purpose

   FAK fret sensor with 20 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-Cer.zf1

   Plasmid

   #61389

   Purpose

   mRNA synthesis of cerulean codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cer.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA-FRT-FAK0

   Plasmid

   #45047

   Purpose

   FAK fret sensor without helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNUT3

   Plasmid

   #175744

   Purpose

   Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

   Depositor

   Yubin Zhou

   Article

   Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

   Insert

   mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-pOTC-3H-GFP

   Plasmid

   #205725

   Purpose

   Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   pOTC-3H-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PH-dL5**

   Plasmid

   #96946

   Purpose

   PLC pleckstrin homology domain linked to FAP for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Use

   Tags

   dL5 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos3.2-LaminA-C-18

   Plasmid

   #57463

   Purpose

   Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. NLS-FUS-Citrine-PixE

   Plasmid

   #111506

   Purpose

   For the nuclear localization of the PixELL system

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

   Insert

   PixE (slr1693 )
   

   Use

   Lentiviral

   Tags

   Citrine, FUS, and NLS

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CHD1 (CHD1A-c107)

   Plasmid

   #110253

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CHD1 (CHD1 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15-MTFmt

    Plasmid

    #31174

    Depositor

    Linda Spremulli

    Article

    Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

    Insert

    Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
    

    Use

    Tags

    His and thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_275

    Plasmid

    #179091

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME nls-eos

    Plasmid

    #73791

    Purpose

    Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

    Depositor

    David Raible

    Article

    Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

    Insert

    nls-eos
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCY 3010-02

    Plasmid

    #36210

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yeast-enhanced (yE) polylinker
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-hphMX4

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVITRO1-iRFP702-LaminB1

    Plasmid

    #184456

    Purpose

    Nuclear membrane-targeted iRFP702 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    iRFP702-LaminB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046538

    Plasmid

    #149128

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ MBIC5-mCer3

    Plasmid

    #74116

    Purpose

    For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

    Depositor

    Michael Tsang

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    MBIC5-mCer3
    

    Use

    Transgenic construct

    Tags

    mCerulean 3

    Expression

    Mutation

    Promoter

    Myl7 (Cmlc2)

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-puro-NLS-LSSmKate1

    Plasmid

    #31873

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4252845

    Plasmid

    #153787

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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